Potential prognostic value in human breast cancer of cytosolic Nme1 protein detection using an original hen specific antibody
| Autor: | E. Marek, B Marques, Gilles Favre, C Toulas, C de Balincourt, J Mihura, Henri Roché, G Soula |
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| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: |
Cancer Research
Mammary gland Breast Neoplasms Biology Metastasis Cytosol Antibody Specificity Gene expression medicine Animals Humans Neoplasm Metastasis Survival rate Monomeric GTP-Binding Proteins Cancer Genes erbB-2 NM23 Nucleoside Diphosphate Kinases medicine.disease Prognosis Nucleoside-diphosphate kinase Survival Rate Metastasis Suppressor Gene medicine.anatomical_structure Oncology Nucleoside-Diphosphate Kinase Cancer research biology.protein Female Antibody Chickens Research Article Transcription Factors |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| Popis: | The metastasis-suppressor nme gene (also called nm23), first identified in murine melanoma cells, exists as two forms in human: nme1 and nme2. However, only the lack of expression of nme1 has been related to distant metastasis appearance in human breast cancer. The aim of this work was first to raise specific antibodies to allow the analysis of Nme1 and then, with this specific tool, to evaluate the predictive value of Nme1 detection in cytosolic extracts of human breast tumours. We obtained a hen antibody that specifically reacts with Nme1 without any cross-reaction with Nme2. We analysed the expression of the protein in 59 human breast tumours and found a significant relationship between this expression and oestrogen receptor status (P |
| Databáze: | OpenAIRE |
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