Lucilia sericata Larval Secretions Stimulating Wound Healing Effects on Rat Dermal Fibroblast Cells
| Autor: | Fahri Akbas, Erdal Polat, İlhan Onaran, Ahmet Ozaydin |
|---|---|
| Přispěvatelé: | İÜC, Cerrahpaşa Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, AKBAŞ, FAHRİ |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Pharmacology
Larva LC-HRMS biology Chemistry Organic Chemistry fungi wound healing Plant Science biology.organism_classification AKBAŞ F. Ozaydin A. Polat E. Onaran I. -Lucilia sericata Larval Secretions Stimulating Wound Healing Effects on Rat Dermal Fibroblast Cells- RECORDS OF NATURAL PRODUCTS cilt.14 ss.340-354 2020 Lucilia Microbiology Dermal fibroblast L. sericata larval secretion Drug Discovery fumaric acid GC-MS Wound healing ferulic acid |
| Popis: | Ozaydin, Ahmet/0000-0003-3959-3053; Akbas, Fahri/0000-0002-3837-250X; Onaran, Ilhan/0000-0003-4630-9516 WOS:000531162200004 The extract from larval Lucilia sericata is used clinically to promote wound healing and tissue regeneration. However, its effect and underlying mechanisms on fibroblast cells, which are involved in the wound healing process, are still poorly understood. This study aimed to examine the effects of larval secretions on dermal fibroblast activity and gene expression and to evaluate the wound healing potential of their major components. Primary rat fibroblasts were cultured and treated with larval secretions. Following the treatment, the cells were used to extract RNA for gene profiling. In addition, migration to the injury site was studied with the scratch healing assay. Our results showed that larval secretion accelerated the migration of the fibroblasts compared to the control cells and that several mRNAs were differentially expressed during a period of 72 h incubation. Additionally, we analyzed the chemical composition of larval secretions and showed that fumaric acid, ferulic acid, and p-coumaric acid, which were selected and identified for their major components, enhanced the migration of the fibroblasts. Therefore, these results indicate that L. sericata larval secretions could modulate the mRNA expression of some wound healing-related genes of the fibroblasts and contain the effective components for wound healing. Bezmialem Vakif University Research CouncilBezmialem Vakif University [4.2018/1] This work was supported by the Bezmialem Vakif University Research Council with grant number 4.2018/1. We highly appreciate the efforts of Monica Ann Ozkan, MSN, RN, and CPAN (Bezmialem Vakif University, Turkiye) in the language editing of this paper. |
| Databáze: | OpenAIRE |
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