Transgenic mice replicating hepatitis B virus but lacking expression of the major HBsAg
| Autor: | Jörg Reimann, Robert Weth, Nina K. Mannes, Michael Nassal, Leonie Halverscheid, Kurt Reifenberg, Reinhold Schirmbeck, Ursula Schultz, Manuela Kleinschmidt, Michael Geissler, Hubert E. Blum |
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| Rok vydání: | 2008 |
| Předmět: |
Serum
Hepatitis B virus HBsAg viruses Mice Transgenic Endoplasmic Reticulum Kidney Virus Replication medicine.disease_cause Virus Mice Sex Factors Viral Envelope Proteins Viral entry Virology medicine Animals RNA Messenger Hepatitis B Surface Antigens biology Age Factors Virion virus diseases Hepatitis B medicine.disease biology.organism_classification digestive system diseases Infectious Diseases Liver Viral replication Hepadnaviridae Hepatocytes RNA Viral Viral load |
| Zdroj: | Journal of Medical Virology. 80:583-590 |
| ISSN: | 1096-9071 0146-6615 |
| DOI: | 10.1002/jmv.21115 |
| Popis: | Hepatitis B Virus (HBV) transgenic mice replicating the viral genome at high level but lacking expression of the small envelope protein (HBsAg) have been produced using a terminally redundant viral DNA construct (HBV 1.4). The generation of viable infectious progeny was dependent on sex and age of mice. Viral mRNA was abundant in liver and kidneys and at low levels in other organs of the mice. No viral particles or HBV envelope proteins could be detected in sera of mice. Despite expression of non-secreted LHBs and MHBs proteins in the liver, there was no accumulation of viral particles in the endoplasmic reticulum of hepatocytes and no necroinflammatory hepatitis was observed. Therefore, these mice represent an excellent model for studies of the role of HBsAg in viral assembly, antiviral immune responses, the further understanding of HBV immunopathogenesis, and the development of antiviral vaccines. |
| Databáze: | OpenAIRE |
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