Expression of ras p21 Oncoprotein in Normal and Neoplastic Human Endometrium
| Autor: | Dario Pellizzola, Pierluigi Benedetti-Panici, Giovanni Scambia, Giovannini G, S. Mancuso, Catozzi L, Mariagrazia Distefano, Francesco Battaglia, Gabriella Ferrandina, Adriano Piffanelli |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Blotting Western Estrogen receptor Oncogene Protein p21(ras) Endometrium Progesterone receptor medicine Carcinoma Humans Neoplasm Invasiveness Epidermal growth factor receptor Aged Neoplasm Staging biology Epithelioma business.industry Endometrial cancer Obstetrics and Gynecology Middle Aged Prognosis medicine.disease Immunohistochemistry Endometrial Neoplasms ErbB Receptors Gene Expression Regulation Neoplastic Postmenopause Genes ras medicine.anatomical_structure Premenopause Receptors Estrogen Oncology biology.protein Female Receptors Progesterone business Densitometry |
| Zdroj: | Gynecologic Oncology. 50:339-346 |
| ISSN: | 0090-8258 |
| DOI: | 10.1006/gyno.1993.1222 |
| Popis: | The ras-encoded p21 protein expression was investigated in 18 normal endometrial tissues and in 37 human primary endometrial carcinomas by Western blotting analysis. Scattered p21 levels were found in normal specimens (mean = 1.29 OD; median = 1.10 OD; range = 0.33-2.65). The p21 levels were significantly higher in secretory (mean = 1.99 OD; median = 2.16 OD; range = 0.71-2.65) than in proliferative (mean = 0.97 OD; median = 1.07 OD; range 0.38-1.73) endometrium (P = 0.009) and higher in primary endometrial carcinomas (mean = 2.05 OD; median = 2.04 OD; range 0.21-4.36) than in normal proliferative tissues (P = 0.004). Immunohistochemical analysis showed that most of the tumor cells expressed p21 oncoprotein while the stromal component was unreactive. No correlation between p21 expression and histopathological characteristics of the tumors was observed. Moreover, estrogen receptor (ER)-positive tumors expressed higher p21 levels than did ER-negative tumors (77% vs 33%; P = 0.009). A similar trend, although not statistically significant, was found between p21 values and progesterone receptor expression (74% vs 44%; P = 0.060). On the other side, p21 levels were unrelated to epidermal growth factor receptor levels. Further studies should verify the possible significance of p21 expression in the prognostic characterization of patients with endometrial cancer. |
| Databáze: | OpenAIRE |
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