Atorvastatin protects against contrast-induced acute kidney injury via upregulation of endogenous hydrogen sulfide

Autor: Li Yinglan, Huang Zena, Liao Xin-xue, Lin Jiaqiong, Lin Yan, Long Ming, Li Xiaoyong, Tan Xuexian, Guo Yue
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Male
Contrast medium
Hospitalized patients
Hydrogen sulfide
Atorvastatin
media_common.quotation_subject
030232 urology & nephrology
hydrogen sulfide
Contrast Media
Cystathionine beta-Synthase
Endogeny
Apoptosis
030204 cardiovascular system & hematology
Pharmacology
Sulfides
Critical Care and Intensive Care Medicine
lcsh:RC870-923
Kidney
Protective Agents
Rats
Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Downregulation and upregulation
Laboratory Study
Medicine
Contrast (vision)
Animals
media_common
Inflammation
business.industry
Acute kidney injury
Cystathionine gamma-Lyase
General Medicine
atorvastatin
medicine.disease
lcsh:Diseases of the genitourinary system. Urology
equipment and supplies
Rats
Up-Regulation
Disease Models
Animal
Oxidative Stress
chemistry
acute kidney injury
Nephrology
business
medicine.drug
Zdroj: Renal Failure
Renal Failure, Vol 42, Iss 1, Pp 270-281 (2020)
ISSN: 1525-6049
0886-022X
Popis: Background: Contrast-induced acute kidney injury (CIAKI) is the third leading cause of acute renal failure in hospitalized patients. This study was aimed to investigate whether atorvastatin could upregulate the expression of hydrogen sulfide (H2S) and hence protect against CIAKI. Methods: We treated male rats and NRK-52E cells by iopromide to establish in vivo and in vitro models of CIAKI. Pretreatment with atorvastatin was given in CIAKI rats to investigate its effect on CIAKI. We collected serum and urine samples to detect renal function. We obtained kidney tissue for histological analysis and detection of protein concentration. We tested the serum concentration of H2S and renal expression of two H2S synthetases [cystathionine γ-lyase (CSE) and cystathionine-β synthase (CBS)]. NaHS was pretreated in NRK-52E cells to testify its underlying effect on contrast-induced injury. Results: Atorvastatin significantly ameliorated renal dysfunction and morphological changes in CIAKI rats, as well as inflammation, apoptosis, and excessive oxidative stress. Atorvastatin also markedly increased the serum concentration of H2S and renal expression of CSE and CBS. Moreover, pretreatment with NaHS in NRK-52E cells considerably attenuated contrast-induced cell death and inflammation. Conclusion: Atorvastatin protects against CIAKI via upregulation of endogenous hydrogen sulfide.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje