In vitro detection of contact allergens: development of an optimized protocol using human peripheral blood monocyte-derived dendritic cells
| Autor: | Andreas Schepky, Klaus-Peter Wittern, Walter Diembeck, Wolfgang Pape, Silke Gerlach, Kerstin Reisinger, Horst Wenck, Jochem Spieker, Ursula Engels, Hendrik Reuter, Ludger Kolbe, Robert Dr. Schmucker |
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| Rok vydání: | 2010 |
| Předmět: |
Cell Survival
Lipopolysaccharide Receptors Cell Separation Cosmetics Toxicology medicine.disease_cause Animal Testing Alternatives Monocytes Toxicity Tests medicine Humans Allergic contact dermatitis Sensitization Cells Cultured Local lymph node assay business.industry In vitro toxicology Reproducibility of Results Cell Differentiation General Medicine Dendritic cell Dendritic Cells Allergens medicine.disease Flow Cytometry In vitro medicine.anatomical_structure Animal Testing Alternative Allergic response Immunology Dermatitis Allergic Contact Cytokines B7-2 Antigen business Haptens |
| Zdroj: | Toxicology in vitro : an international journal published in association with BIBRA. 25(1) |
| ISSN: | 1879-3177 |
| Popis: | Allergic contact dermatitis is a delayed T-cell mediated allergic response associated with relevant social and economic impacts. Animal experiments (e.g. the local lymph node assay) are still supplying most of the data used to assess the sensitization potential of new chemicals. However, the 7th amendment to the EU Cosmetic Directive will introduce a testing ban for cosmetic ingredients after 2013. In vitro alternative methods are thus being actively developed. Although promising results have been obtained with cell lines, their reduced functionality and inherent genomic instability led us to reinvestigate the use of peripheral blood monocyte-derived dendritic cells (PBMDCs) for the establishment of a reliable in vitro sensitization test. To solve the issues associated with the use of primary cells, the culture and exposure conditions (cytokine concentrations, incubation time, readout, pooled vs. single donors and cytotoxicity) were re-assessed and optimized. Here we propose a stable and reproducible protocol based on PBMDCs. This should allow a wider acceptance of PBMDCs as a reliable test system for the detection of human skin sensitizers and the inclusion of this protocol in an integrated testing strategy. |
| Databáze: | OpenAIRE |
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