Preferential orientation of an immunoglobulin in a glycolipid monolayer controlled by the disintegration kinetics of proteo-lipidic vesicles spread at an air–buffer interface
| Autor: | Stéphanie Godoy, Pierre R. Coulet, Agnès Girard-Egrot, Paul Boullanger, Jean Paul Chauvet |
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| Jazyk: | angličtina |
| Předmět: |
Monoclonal antibody
Membrane Fluidity Protein Conformation Surface Properties Kinetics Biophysics Biocompatible Materials Biosensing Techniques Glycolipid Buffers Biochemistry Diffusion Monolayer Molecule Surface Tension Vesicle spreading Chromatography Interfacial monolayer Chemistry Vesicle Air Membranes Artificial Cell Biology Solutions Ionic strength Immunoglobulin G Liposomes Compressibility Acetylcholinesterase Glycolipids Protein orientation Saturation (chemistry) |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Biomembranes. (1-2):39-51 |
| ISSN: | 0005-2736 |
| DOI: | 10.1016/j.bbamem.2003.09.001 |
| Popis: | The insertion of immunoglobulin (IgG) in a glycolipid monolayer was achieved by using the ability of new proteo-glycolipid vesicles to disintegrate into a mixed IgG–glycolipid interfacial film after spreading at an air–buffer interface. The interfacial disintegration kinetics was shown to be directly dependent on the initial vesicle surface density and on the buffer ionic strength. The presence of the immunoglobulin in the glycolipid film was displayed by an increase of the lateral compressibility (Cs) during monolayer compression. Cs magnitude modifications, due to the antibody effect on the monolayer packing, decreases as the spread vesicle density increases. At interfacial saturation, the lateral compressibility profile becomes similar to that of a control monolayer without antibody. However, the careful analysis of the mixed monolayer after transfer by Langmuir–Blodgett technique (ATR-FTIR characterisation, enzyme immunoassociation) clearly demonstrated that the antibody was still present in such conditions and was not completely squeezed out from the interface as compressibility changes could have meant. At nonsaturating vesicle surface density, IgG molecules initially lying in the lipid matrix with the Y-shape plane parallel to the interface move to a standing-up position during the compression, leading to lateral compressibility modifications. For a saturating vesicle surface density, the glycolipid molecules force the IgG molecules to directly adopt a more vertical position in the interfacial film and, consequently, no lateral compressibility modification was recorded during the compression. |
| Databáze: | OpenAIRE |
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