Immunometabolic phenotype of BV-2 microglia cells upon murine cytomegalovirus infection

Autor: Valentino Rački, Marina Marcelić, Kristina Jurdana, Kristina Grabušić, Natalia Kučić
Rok vydání: 2018
Předmět:
0301 basic medicine
Muromegalovirus
Nitric Oxide Synthase Type II
Culture Media
Serum-Free
Mice
0302 clinical medicine
Glycolysis
Mice
Inbred BALB C
Microglia
Murine cytomegalovirus
BV-2 cells
Herpesviridae Infections
Phenotype
Cell biology
Nitric oxide synthase
medicine.anatomical_structure
Neurology
Host-Pathogen Interactions
BIOMEDICINA I ZDRAVSTVO. Temeljne medicinske znanosti
Mannose receptor
Mannose Receptor
Signal Transduction
Primary Cell Culture
Congenital cytomegalovirus infection
Receptors
Cell Surface
Biology
Models
Biological
Cell Line
03 medical and health sciences
Cellular and Molecular Neuroscience
In vivo
Virology
medicine
Animals
Lectins
C-Type
Arginase
BIOMEDICINE AND HEALTHCARE. Basic Medical Sciences
Fibroblasts
medicine.disease
Embryo
Mammalian
Hypoxia-Inducible Factor 1
alpha Subunit
030104 developmental biology
Mannose-Binding Lectins
Gene Expression Regulation
biology.protein
Neurology (clinical)
030217 neurology & neurosurgery
Homeostasis
Zdroj: Journal of neurovirology
Volume 25
Issue 4
ISSN: 1538-2443
1355-0284
Popis: Microglia are resident brain macrophages with key roles in development and brain homeostasis. Cytomegalovirus (CMV) readily infects microglia cells, even as a possible primary target of infection in development. Effects of CMV infection on a cellular level in microglia are still unclear ; therefore, the aim of this research was to assess the immunometabolic changes of BV-2 microglia cells following the murine cytomegalovirus (MCMV) infection. In light of that aim, we established an in vitro model of ramified BV-2 microglia (BV-2∅FCS, inducible nitric oxide synthase (iNOSlow), arginase-1 (Arg-1high), mannose receptor CD206high, and hypoxia-inducible factor 1α (HIF-1αlow)) to better replicate the in vivo conditions by removing FCS from the cultivation media, while the cells cultivated in 10% FCS DMEM displayed an ameboid morphology (BV-2FCS high, iNOShigh, Arg-1low, CD206low, and HIF- 1αhigh). Experiments were performed using both ramified and ameboid microglia, and both of them were permissive to productive viral infection. Our results indicate that MCMV significantly alters the immunometabolic phenotypic properties of BV-2 microglia cells through the manipulation of iNOS and Arg-1 expression patterns, along with an induction of a glycolytic shift in the infected cell cultures.
Databáze: OpenAIRE
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