Expression of tumor-suppressor genes interferon regulatory factor 1 and death-associated protein kinase in primitive acute myelogenous leukemia cells

Autor: Craig T. Jordan, Monica L. Guzman, Barry Grimes, David A. Rizzieri, Gordon L. Phillips, Selina M. Luger, Dianna S. Howard, Donna Upchurch
Rok vydání: 2001
Předmět:
Tumor suppressor gene
Immunology
Antigens
CD34
Apoptosis
Biology
medicine.disease_cause
Biochemistry
Immunophenotyping
NAD+ Nucleosidase
Antigens
CD
hemic and lymphatic diseases
Complementary DNA
medicine
Humans
Genes
Tumor Suppressor
ADP-ribosyl Cyclase
Regulation of gene expression
Membrane Glycoproteins
Gene Expression Regulation
Leukemic
Reverse Transcriptase Polymerase Chain Reaction
Cell Biology
Hematology
Hematopoietic Stem Cells
Phosphoproteins
medicine.disease
ADP-ribosyl Cyclase 1
Antigens
Differentiation
Neoplasm Proteins
DNA-Binding Proteins
Death-Associated Protein Kinases
Leukemia
IRF1
Leukemia
Myeloid
Enzyme Induction
Acute Disease
Calcium-Calmodulin-Dependent Protein Kinases
Neoplastic Stem Cells
Cancer research
Stem cell
Apoptosis Regulatory Proteins
Carcinogenesis
Interferon Regulatory Factor-1
Zdroj: Blood. 97:2177-2179
ISSN: 1528-0020
0006-4971
DOI: 10.1182/blood.v97.7.2177
Popis: Previous studies indicate that human acute myelogenous leukemia (AML) arises from a rare population of leukemic stem cells. Cells of this nature can initiate and maintain leukemic cell growth in both long-term cultures and nonobese diabetic/severe combined immune-deficient mice. To characterize the biology of primitive AML cells, gene expression screens were performed with 7 primary AML and 3 normal specimens. For each sample, stem cell populations (CD34+/CD38−) were isolated and used to synthesize radiolabeled complementary DNA (cDNA). AML vs normal probes were then hybridized to cDNA arrays containing genes related to cancer and apoptosis. Of approximately 1400 genes analyzed, 2 tumor-suppressor genes were identified that were overexpressed in all 7 of the AML CD34+/CD38−cell populations: death-associated protein kinase and interferon regulatory factor 1. Expression of each gene was confirmed by reverse-transcription polymerase chain reaction and immunoblot analysis. It is proposed that tumor-suppressor proteins play a role in the biology of primitive AML cells.
Databáze: OpenAIRE
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