Calcium-dependent aggregation and fusion of phosphatidylcholine liposomes induced by complexes of flavonoids with divalent iron
| Autor: | Bolatbek S. Abdrasilov, Eugeny N. Muzafarov, Yuri A. Kim, Elena A. Yagolnik, Yury S. Tarahovsky |
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| Rok vydání: | 2012 |
| Předmět: |
Cations
Divalent Iron Biophysics Membrane fusion Morin Biochemistry Divalent chemistry.chemical_compound Phosphatidylcholine Fluorescence Resonance Energy Transfer Taxifolin heterocyclic compounds Lipid bilayer Flavonoids chemistry.chemical_classification Liposome Membrane adhesion Vesicle food and beverages Lipid bilayer fusion Cell Biology chemistry Liposomes Phosphatidylcholines Flavonoid Calcium |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Biomembranes. 1818:695-702 |
| ISSN: | 0005-2736 |
| DOI: | 10.1016/j.bbamem.2011.12.001 |
| Popis: | It was found that complexes of the flavonoids quercetin, taxifolin, catechin and morin with divalent iron initiated an increase in light scattering in a suspension of unilamellar 100 nm liposomes. The concentration of divalent iron in the suspension was 10 μM. Liposomes were prepared from 1-palmitoyl-2-oleoylglycero-3-phoshpatidylcholine. The fluorescent resonance energy transfer (FRET) analysis of liposomes labeled with NBD-PE and lissamine rhodamine B dyes detected a slow lipid exchange in liposomes treated with flavonoid-iron complexes and calcium, while photon correlation spectroscopy and freeze-fracture electron microscopy revealed the aggregation and fusion of liposomes to yield gigantic vesicles. Such processes were not found in liposomes treated with phloretin because this flavonoid is unable to interact with iron. Rutin was also unable to initiate any marked changes because this water-soluble flavonoid cannot interact with the lipid bilayer. The experimental data and computer calculations of lipophilicity (cLogP) as well as the charge distribution on flavonoid-iron complexes indicate that the adhesion of liposomes is provided by an iron link between flavonoid molecules integrated in adjacent bilayers. It is supposed that calcium cations facilitate the aggregation and fusion of liposomes because they interact with the phosphate moieties of lipids. |
| Databáze: | OpenAIRE |
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