Liquid chromatography–electrospray mass spectrometry determination of carbamazepine, oxcarbazepine and eight of their metabolites in human plasma
| Autor: | Marylène Cociglio, Dominique Hillaire-Buys, J.-P. Blayac, Hélène Breton, Hélène Peyrière, Françoise Bressolle |
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| Přispěvatelé: | Dynamique des capacités humaines et des conduites de santé (EPSYLON), Université de Montpellier (UM)-Université Paul-Valéry - Montpellier 3 (UPVM)-Université Montpellier 1 (UM1) |
| Rok vydání: | 2005 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Electrospray Metabolite Clinical Biochemistry Oxcarbazepine Mass spectrometry Sensitivity and Specificity 030226 pharmacology & pharmacy 01 natural sciences Biochemistry Mass Spectrometry Analytical Chemistry Acetone 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Liquid chromatography–mass spectrometry medicine Chemical Precipitation Humans Quadrupole mass analyzer Chromatography medicine.diagnostic_test 010401 analytical chemistry Reproducibility of Results Cell Biology General Medicine Reference Standards 0104 chemical sciences Carbamazepine chemistry Therapeutic drug monitoring Drug Monitoring Quantitative analysis (chemistry) [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology Chromatography Liquid medicine.drug |
| Zdroj: | Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, Elsevier, 2005, 828 (1-2), pp.80-90 |
| ISSN: | 1570-0232 1873-376X |
| DOI: | 10.1016/j.jchromb.2005.09.019 |
| Popis: | International audience; Carbamazepine (CBZ) and oxcarbazepine (OXCBZ) are both antiepileptic drugs, which are prescribed as first-line drugs for the treatment of partial and generalized tonic–clonic epileptic seizures. In this paper, a specific and sensitive liquid chromatography–electrospray ionization mass spectrometry method was described for the simultaneous determination of carbamazepine (CBZ), oxcarbazepine (OXCBZ) and eight of their metabolites [CBZ-10,11-epoxide (CBZ-EP), 10,11-dihydro-10,11-trans-dihydroxy-carbamazepine (DiOH-CBZ), 10-hydroxy-10,11-dihydroCBZ (10-OH-CBZ), 2-hydroxycarbamazepine (2-OH-CBZ), 3-hydroxycarbamazepine (3-OH-CBZ), iminostilbene (IM), acridone (AO) and acridine (AI)] in human plasma. The work-up procedure involved a simple precipitation with acetone. Separation of the analytes was achieved within 50 min using a Zorbax eclipse XD8 C8 analytical column. The mobile phase consisted of a mixture of acetonitrile–formate buffer (2 mM, pH 3). Detection was performed using a quadrupole mass spectrometer fitted with an electrospray ion source. Mass spectrometric data were acquired in single ion recording mode at m/z 237 for CBZ, m/z 180 for CBZ-EP and AI, m/z 236 for OXCBZ, m/z 237 for 10-OH-CBZ, m/z 253 for 2-OH-CBZ, 3-OH-CBZ and DiOH-CBZ, m/z 196 for AO and m/z 194 for IM. For all analytes, the drug/internal standard peak height ratios were linked via a quadratic relationship to plasma concentrations. The extraction recovery averaged 90% for CBZ, 80% for OXCBZ and was 80–105% for the metabolites. The lower limit of quantitation was 0.5 mg/l for CBZ, 0.4 mg/l for OXCBZ and ranged from 0.02 to 0.3 mg/l for the metabolites. Precision ranged from 2 to 13% and accuracy was between 86 and 112%. This method was found suitable for the analysis of plasma samples collected during therapeutic drug monitoring of patients treated with CBZ or OXCBZ. |
| Databáze: | OpenAIRE |
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