The use of two-dimensional electrophoresis to detect mutations induced in mouse spermatogonia by ethylnitrosourea
Autor: | A. S. Raj, J. A. Heddle, R. R. Marshall, F. J. Grant |
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Rok vydání: | 1983 |
Předmět: |
Male
Mutation rate Locus (genetics) Mutagen Plant Science Biology medicine.disease_cause Nitrosourea Compounds Mice In vivo Genetics medicine Animals Isoelectric Point Gene Proteins Cell Biology Spermatozoa Molecular biology Spermatogonia Molecular Weight Electrophoresis medicine.anatomical_structure Liver Biochemistry Ethylnitrosourea Mutation Gamete Electrophoresis Polyacrylamide Gel |
Zdroj: | Canadian Journal of Genetics and Cytology. 25:457-466 |
ISSN: | 0008-4093 |
DOI: | 10.1139/g83-070 |
Popis: | Two-dimensional electrophoresis should, in theory, be a suitable method for the measurement of induced mutation rates in the germ cells of mice. Not only can the polypeptide products of a large numer of genes be resolved on a single gel but the detection of mutations which lead to proteins with altered electrophoretic properties (but not necessarily altered function) is possible. Our attempts to apply two-dimensional electrophoresis to the detection of mutation in vivo have involved three stages: (i) the rapid production of gels of high resolution and reproducibility; (ii) the identification of eight interstrain protein variants and demonstration of their simple genetic basis; and (iii) a pilot experiment using the powerful germ-cell mutagen ethylnitrosourea. It was found that although interstrain protein variants could be detected and shown to be inherited in a codominant manner, induced variants were rarely detected even on high quality gels. Only 2 variants were detected among 67 offspring of male mice treated with 150 mg/kg ethylnitrosourea. This represented a mutation rate of 0.88 × 10−4 mutations per locus per gamete. |
Databáze: | OpenAIRE |
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