Optimal Aspergillus fumigatus and Asp f 1 serum IgG cut-offs for the diagnosis of allergic bronchopulmonary aspergillosis
| Autor: | Tadashi Nagai, Eiji Nakatani, Akemi Saito, Yoshiki Shiraishi, Koichiro Asano, Yuto Hamada, Masami Taniguchi, Kiyoshi Sekiya, Tsuyoshi Oguma, Kazuki Harada, Yosuke Kamide, Yuma Fukutomi, Kentaro Watai |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
lcsh:Immunologic diseases. Allergy 0301 basic medicine Antigens Fungal IgG Aspergillus fumigatus law.invention Precipitin 03 medical and health sciences 0302 clinical medicine Reference Values law Allergic bronchopulmonary aspergillosis medicine Humans Immunology and Allergy Aged Asthma biology medicine.diagnostic_test business.industry Aspergillosis Allergic Bronchopulmonary Serine Endopeptidases Cut-off General Medicine Allergens Immunoglobulin E Middle Aged biology.organism_classification medicine.disease Asp f 1 030104 developmental biology ROC Curve 030228 respiratory system IgG binding Case-Control Studies Immunoglobulin G Immunoassay Immunology Recombinant DNA biology.protein Antibody lcsh:RC581-607 business Biomarkers |
| Zdroj: | Allergology International, Vol 70, Iss 1, Pp 74-80 (2021) |
| ISSN: | 1323-8930 |
| Popis: | Background: The presence of IgG antibodies (Abs) to Aspergillus fumigatus (Af) is a crucial diagnostic criterion for allergic bronchopulmonary aspergillosis (ABPA). Although precipitation is traditionally used to document IgG Abs, anti-Af serum IgG levels can also be measured by enzyme immunoassay (EIA). However, there are insufficient data on the optimal cut-offs to assess diagnostic performance of the EIA method. This study aimed to determine cut-off levels of IgG binding crude Af extracts or recombinant Asp f 1 (by ImmunoCAP®) and to compare their efficacy for ABPA diagnosis with Af-precipitating Abs. Methods: The age distribution of levels of IgG to crude extracts of Af (Af-IgG) and recombinant Asp f 1 (Asp f 1-IgG) was established using sera from 694 healthy controls (HC). Receiver operating characteristic analysis for Af-IgG and Asp f 1-IgG levels for the purpose of ABPA diagnosis was performed in 306 Af-sensitized asthma patients (including 49 ABPA), and cut-offs were determined. Results: An age-dependent decline in the levels of Af-IgG was observed in HC. Thus, cut-offs for Af-IgG levels were determined separately by age as 60 mg/L for patients aged |
| Databáze: | OpenAIRE |
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