Escherichia coli promotes macrophage apoptosis
| Autor: | Pravin C. Singhal, Konstantinos G. Stravodimos, Saroj Sharma, Arthur D. Smith, Krishna Reddy |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Necrosis
Urology Virulence Apoptosis Cell Count DNA Fragmentation medicine.disease_cause Peripheral blood mononuclear cell Monocytes Microbiology Cicatrix Mice medicine Escherichia coli Animals Humans Escherichia coli Infections Electrophoresis Agar Gel business.industry Monocyte DNA U937 Cells Fibrosis In vitro medicine.anatomical_structure Cell culture Immunology Urinary Tract Infections Macrophages Peritoneal medicine.symptom business |
| Zdroj: | Journal of endourology. 13(4) |
| ISSN: | 0892-7790 |
| Popis: | Background: Escherichia coli is the bacterium most commonly isolated from the urine of patients with urinary tract infection (UTI). Recurrent episodes of UTI lead to renal interstitial scarring. In interstitial fibrosis and scarring, infiltration of mononuclear cells has been reported to play a key role. Materials and Methods: We evaluated the effect of two strains of E. coli—the pathogenic BH-5 and the plasmidless, nonfimbriated HB-101–on human monocyte and murine macrophage apoptosis. Results: E. coli BH-5 enhanced apoptosis in a time- and dose-dependent manner. It also promoted necrosis in a time- and dose-dependent manner. Strain HB-101 promoted monocyte apoptosis in a dose-dependent manner. However, the magnitude of HB-101-induced monocyte apoptosis was lower than BH-5-induced macrophage apoptosis. Conclusion: The ability of E. coli to induce apoptosis may contribute to its virulence and play a role in renal interstitial scarring. |
| Databáze: | OpenAIRE |
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