| Autor: |
Kunfeng, Zhang, Yuzhe, Huang, Qianzhen, Wu, Wenhua, Guo, Hao, Chen, Wenna, Zhang, Yong, Li, Yongming, Lu, Qingxi, Wu, Wenjuan, Pan, Lei, Chen, Yan, Chen |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
International Journal of Biological Macromolecules. 207:750-759 |
| ISSN: |
0141-8130 |
| Popis: |
The objectives of this study were (1) to prepare Armillariella tabescens mycelia polysaccharides (PAT) with remarkably growth inhibitory effect on typical food-borne pathogenic bacteria using a green and efficient polyamide method and (2) to explore the antibacterial mechanism of PAT for use as a natural antibacterial agent. The sugar and uronic acid contents of PAT were 93.41% and 12.24%, respectively. PAT could inhibit the growth of Escherichia coli, Proteus vulgaris, Bacillus subtilis, and Staphylococcus aureus cells, with minimum inhibitory concentrations of 0.5, 1.0, 4.0, and 4.0 mg/mL, respectively. Ultra-high-resolution field emission scanning electron microscopy and high-resolution transmission electron microscopy analysis revealed cell wall and membrane rupture of E. coli treated with PAT. Further, 0.5-4.0 mg/mL PAT was found to significantly (P 0.01) and concentration-dependently increase the conductivity of the broth, exudation of the intracellular protein, and alkaline phosphatase and β-galactosidase activities. Confocal laser scanning microscopy revealed morphological changes in E. coli DNA after PAT treatment and intracellular reactive oxygen species accumulation; flow cytometry revealed E. coli cell apoptosis. Our findings provide a theoretical basis and technical support for the development of PAT as a natural antibacterial product. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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