Vdr expression in osteoclast precursors is not critical in bone homeostasis
| Autor: | Annemieke Verstuyf, Iris Janssens, Geert Carmeliet, Stefanie Doms, Justine Vanhevel, Lieve Verlinden |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
musculoskeletal diseases
0301 basic medicine medicine.medical_specialty bone homeostasis Endocrinology Diabetes and Metabolism Clinical Biochemistry Osteoclasts chemistry.chemical_element Mice Transgenic Calcium Biochemistry Calcitriol receptor Bone and Bones Bone remodeling 03 medical and health sciences 0302 clinical medicine Endocrinology Osteoclast Internal medicine medicine Animals Homeostasis vitamin D receptor Molecular Biology Cells Cultured Calcium metabolism Osteoblasts biology Cell Biology Coculture Techniques Calcium Dietary osteoclasts 030104 developmental biology medicine.anatomical_structure chemistry RANKL 030220 oncology & carcinogenesis Macrophages Peritoneal biology.protein Receptors Calcitriol Molecular Medicine Female Cortical bone |
| Zdroj: | The Journal of Steroid Biochemistry and Molecular Biology. 195:105478 |
| ISSN: | 0960-0760 |
| Popis: | The long-recognized role of the vitamin D endocrine system is to maintain stable serum calcium concentrations, which are ensured by a complex interplay between parathyroid gland, kidney, intestine, and bone. However, although VDR is expressed in osteoclastogenic cells, the contribution of VDR-mediated signaling to osteoclast differentiation and activity remains undefined. We therefore deleted Vdr expression efficiently and specifically in myeloid cells by use of M lysozyme-driven Cre expression, which targets granulocytes, monocytes, macrophages and osteoclasts (Vdrmyel- mice). Bone and calcium homeostasis were investigated under basal conditions and in conditions of increased bone remodeling, by feeding Vdrmyel- and Vdrmyel+ (wildtype) mice either a normal (1%) or a low (0.02%) calcium diet from weaning onwards. Vdrmyel- mice developed normally and were normocalcemic at the age of 8 weeks, both at the normal and the low calcium diet. No differences in trabecular or cortical bone mass were observed between Vdrmyel- mice and their wildtype littermates. Dietary calcium restriction resulted in a comparable reduction of trabecular bone mass (40%) and cortical thickness (48%) in Vdrmyel- and Vdrmyel+ mice, pointing to a massive transfer of calcium from the bone to the serum. In agreement with these results, osteoclastic differentiation of hematopoietic cells of Vdrmyel- mice, either induced by M-CSF and RANKL, or cocultured with osteoblasts, occurred as efficiently as osteoclastogenesis from Vdrmyel+ mice. In conclusion, our data do not support a role for osteoclastic Vdr signaling in the control of bone homeostasis. ispartof: JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY vol:195 ispartof: location:England status: published |
| Databáze: | OpenAIRE |
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