Transcript profiling of chickpea pod wall revealed the expression of floral homeotic gene AGAMOUS-like X2 (CaAGLX2)
| Autor: | Mamta Bhattacharjee, Jagadale Mahesh Vasantrao, Sumita Acharjee, Debashis Panda, Bidyut Kumar Sarmah, Indrani K. Baruah |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Gene isoform MADS Domain Proteins Flowers Biology 03 medical and health sciences 0302 clinical medicine Gene Expression Regulation Plant Gene expression Genetics Molecular Biology Gene Plant Proteins Expressed sequence tag Subtractive Hybridization Techniques Agamous food and beverages Computational Biology General Medicine Cicer 030104 developmental biology Point of delivery Suppression subtractive hybridization 030220 oncology & carcinogenesis Seeds Homeotic gene Transcriptome |
| Zdroj: | Molecular biology reports. 46(6) |
| ISSN: | 1573-4978 |
| Popis: | The differentially expressed genes in the chickpea pod wall have been identified for the first time using a forward suppression subtractive hybridization (SSH) library. In all, 226 clones of SSH library were sequenced and analyzed. A total of 179 high-quality expressed sequence tags (ESTs) were generated and based on the CAP3 assembly of these ESTs, 126 genes (97 singletons and 29 contigs) were computationally annotated. The mapping of 88.26% ESTs by gene ontology (GO) annotation distributed them into 751 GO terms of three categories, cellular location, molecular function, and biological process. The KEGG pathway analysis revealed 45 ESTs are involved in 49 different biological pathways. Also, 67 ESTs encodes four different classes of enzymes such as oxidoreductases (29), transferase (20), hydrolases (16) and isomerase (2). Six genes were selected and subjected to qPCR analysis, of these, two genes (FHG Floral homeotic AGAMOUS-like isoform X2, MADS1 MADS-box transcription factor) showed significant up-regulation in the pod wall compared to leaves. Surprisingly, one of the MADS1 box gene, FHG (CaAGLX2), responsible for flower development expressed in the pod wall. Therefore, understanding its specific role in the pod wall could be interesting. Thus, the transcript dynamics of the chickpea pod wall revealed differentially expressed genes in the pod wall, which may be participating in the metabolic build-up of both pod wall and seeds. |
| Databáze: | OpenAIRE |
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