Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification
| Autor: | Greg M. Harris, Jacob A. Orkwis, Zhenyuan Xu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
General Chemical Engineering Schwann cell General Biochemistry Genetics and Molecular Biology Extracellular matrix 03 medical and health sciences 0302 clinical medicine medicine Animals Cell Proliferation Extracellular Matrix Proteins General Immunology and Microbiology Cell growth Chemistry Regeneration (biology) General Neuroscience Phenotype Extracellular Matrix Cell biology 030104 developmental biology medicine.anatomical_structure nervous system 030220 oncology & carcinogenesis Peripheral nervous system Schwann Cells Function (biology) Micropatterning |
| Zdroj: | Journal of Visualized Experiments. |
| ISSN: | 1940-087X |
| DOI: | 10.3791/61496-v |
| Popis: | Traumatic peripheral nervous system (PNS) injuries currently lack suitable treatments to regain full functional recovery. Schwann cells (SCs), as the major glial cells of the PNS, play a vital role in promoting PNS regeneration by dedifferentiating into a regenerative cell phenotype following injury. However, the dedifferentiated state of SCs is challenging to maintain through the time-period needed for regeneration and is impacted by changes in the surrounding extracellular matrix (ECM). Therefore, determining the complex interplay between SCs and differing ECM to provide cues of regenerative potential of SCs is essential. To address this, a strategy was created where different ECM proteins were adsorbed onto a tunable polydimethylsiloxane (PDMS) substrate which provided a platform where stiffness and protein composition can be modulated. SCs were seeded onto the tunable substrates and critical cellular functions representing the dynamics of SC phenotype were measured. To illustrate the interplay between SC protein expression and cellular morphology, differing seeding densities of SCs in addition to individual microcontact printed cellular patterns were utilized and characterized by immunofluorescence staining and western blot. Results showed that cells with a smaller spreading area and higher extent of cellular elongation promoted higher levels of SC regenerative phenotypic markers. This methodology not only begins to unravel the significant relationship between the ECM and cellular function of SCs, but also provides guidelines for the future optimization of biomaterials in peripheral nerve repair. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|