Ascaris lumbricoides eggs or artefacts? A diagnostic conundrum
| Autor: | Davide Ianniello, M.P. Maurelli, Antonio Montresor, C. S. Aggarwal, L. Gualdieri, Bruno Levecke, Leucio Camara Alves, Piet Cools, Giuseppe Cringoli, Paola Cociancic, Laura Rinaldi |
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| Přispěvatelé: | Maurelli, M. P., Alves, L. C., Aggarwal, C. S., Cociancic, P., Levecke, B., Cools, P., Montresor, A., Ianniello, D., Gualdieri, L., Cringoli, G., Rinaldi, L. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Veterinary medicine
Stool sample decorticated eggs HELMINTH PARASITES artefacts decorticated eggs FLOTAC THICK SMEAR fluids and secretions artefacts decorticated egg Mini-FLOTAC misdiagnosis Ascaris lumbricoides artefacts biology Biology and Life Sciences SUUM EGGS biology.organism_classification Molecular analysis Infectious Diseases Kato-katz INFECTIONS Kato katz Ascaris lumbricoide Animal Science and Zoology Parasitology Research Article |
| Zdroj: | PARASITOLOGY Parasitology |
| ISSN: | 0031-1820 1469-8161 |
| Popis: | Due to the presence of artefacts in stool samples, the copromicroscopic diagnosis ofAscaris lumbricoidesis not always straightforward, particularly in the case of fertilized decorticated eggs. A total of 286 stool samples from 115 schoolchildren in India and 171 adult immigrants in Italy were screened for the presence ofA. lumbricoideseggs by both Kato-Katz thick smear and Mini-FLOTAC. If the outer layer ofA. lumbricoideseggs was absent, two aliquots of each stool sample were preserved: one for coproculture to identify larvae after development and one to compose a pool of stool for molecular analysis. A total of 64 stool samples (22.4%) were positive forA. lumbricoidesusing the Kato-Katz thick smear; 36 (56.3%) of these showed mammillatedA. lumbricoideseggs, 25 (39.1%) showed elements resembling fertilized decorticated eggs, while three samples (4.7%) showed both mammillated and decorticated eggs. By Mini-FLOTAC, 39 stool samples (13.6%) were positive, while decorticatedA. lumbricoides-like eggs were identified as artefacts. These results were confirmed by negative coprocultures and quantitative polymerase chain reaction. Mini-FLOTAC can be used for a reliable diagnosis ofA. lumbricoides, thanks to the flotation and translation features which allow a clearer view, resulting in the correct identification ofA. lumbricoideseggs. |
| Databáze: | OpenAIRE |
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