Clearance of chicken cystatin from the rat circulation
| Autor: | Mariusz Siekierka, Jakub Gburek, Jerzy Osada, Maria Warwas |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Male
medicine.medical_specialty Density gradient Metabolic Clearance Rate Physiology Urine Biology Kidney urologic and male genital diseases Biochemistry Internal medicine medicine Animals Tissue Distribution Rats Inbred BUF Molecular Biology Egg Proteins Chicken cystatin Cystatins Rats Endocrinology Organ Specificity Chickens Percoll Half-Life Egg white |
| Zdroj: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 112:697-701 |
| ISSN: | 1096-4959 |
| DOI: | 10.1016/0305-0491(95)00122-0 |
| Popis: | Chicken cystatin (mixed form) was prepared from egg white. Radioactively labeled preparations were administered intravenously to rats. 125 I-cystatin disappeared from the rat circulation with a half-life of -73 min. The radiolabeled inhibitor was rapidly taken up by the kidneys. Percoll density gradient analysis showed that it was incorporated into lysosomes. Within 24 hr after the injection of 125 I-cystatin, 25% of the administered radioactivity was recovered in the urine, but only 2% was in the protein-bound form. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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