Parechovirus A Infection of the Intestinal Epithelium: Differences Between Genotypes A1 and A3
| Autor: | Inés García-Rodríguez, Hetty van Eijk, Gerrit Koen, Dasja Pajkrt, Adithya Sridhar, Katja C. Wolthers |
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| Přispěvatelé: | Graduate School, Medical Microbiology and Infection Prevention, AII - Infectious diseases, Paediatric Infectious Diseases / Rheumatology / Immunology, ARD - Amsterdam Reproduction and Development |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Genotype enteroids Immunology Transwell Picornaviridae human organoids Parechovirus Microbiology Feces Cellular and Infection Microbiology polarized epithelium medicine Humans Respiratory system Intestinal Mucosa Tropism Original Research Picornaviridae Infections biology Human parechovirus fungi PeV-A medicine.disease biology.organism_classification Intestinal epithelium Virology QR1-502 Infectious Diseases Gastrointestinal disease Child Preschool |
| Zdroj: | Frontiers in Cellular and Infection Microbiology Frontiers in cellular and infection microbiology, 11:740662. Frontiers Media S.A. Human Organoid Technology for Virus Research Frontiers in Cellular and Infection Microbiology, Vol 11 (2021) |
| ISSN: | 2235-2988 |
| Popis: | Human parechovirus (PeV-A), one of the species within the Picornaviridae family, is known to cause disease in humans. The most commonly detected genotypes are PeV-A1, associated with mild gastrointestinal disease in young children, and PeV-A3, linked to severe disease with neurological symptoms in neonates. As PeV-A are detectable in stool and nasopharyngeal samples, entry is speculated to occur via the respiratory and gastro-intestinal routes. In this study, we characterized PeV-A1 and PeV-A3 replication and tropism in the intestinal epithelium using a primary 2D model based on human fetal enteroids. This model was permissive to infection with lab-adapted strains and clinical isolates of PeV-A1, but for PeV-A3, infection could only be established with clinical isolates. Replication was highest with infection established from the basolateral side with apical shedding for both genotypes. Compared to PeV-A1, replication kinetics of PeV-A3 were slower. Interestingly, there was a difference in cell tropism with PeV-A1 infecting both Paneth cells and enterocytes, while PeV-A3 infected mainly goblet cells. This difference in cell tropism may explain the difference in replication kinetics and associated disease in humans. |
| Databáze: | OpenAIRE |
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