Cartilage debris increases the expression of chondrodestructive tumor necrosis factor-α by articular chondrocytes
| Autor: | Carmelita G. Frondoza, Michelle Cameron-Donaldson, David S. Hungerford, Christoph Holland |
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| Rok vydání: | 2004 |
| Předmět: |
musculoskeletal diseases
Pathology medicine.medical_specialty Tumor Necrosis Factor-alpha business.industry Vascular disease Cartilage Osteoarthritis medicine.disease Chondrocyte In vitro Reverse transcription polymerase chain reaction Chondrocytes medicine.anatomical_structure Arthropathy medicine Humans Orthopedics and Sports Medicine Tumor necrosis factor alpha business Cells Cultured |
| Zdroj: | Arthroscopy: The Journal of Arthroscopic & Related Surgery. 20:1040-1043 |
| ISSN: | 0749-8063 |
| DOI: | 10.1016/j.arthro.2004.08.009 |
| Popis: | Purpose: To determine if cartilage particles increased the expression of TNF-α by articular chondrocytes. Type of study: In vitro experiment. Methods: Articular chondrocytes were obtained from patients undergoing primary total knee arthroplasty for osteoarthritis (n = 3) and from patients undergoing below-knee amputation for peripheral vascular disease (n = 3). Chondrocytes were then incubated with and without cartilage particles at a concentration of 5 μg/10 5 cells for 24 hours. TNF-α levels were then determined using reverse transcription polymerase chain reaction. Results: Both normal and osteoarthritic chondrocytes had low baseline expression of TNF-α under standard cell culture conditions. Expression was markedly increased in response to incubation with cartilage particles, and was statistically significant. Conclusions: Cartilage debris in the traumatized and osteoarthritic joint may increase the concentration of TNF-α in the joint, contributing to joint symptoms and cartilage destruction. Arthroscopic debridement and lavage may improve symptoms by washing these harmful components from the joint. Level of evidence: Level IV. |
| Databáze: | OpenAIRE |
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