| Autor: |
Chunmei Liao, Hui Yang, Tong Su, Chao Fang, Hao Du, Yanping Wang, Baohui Liu, Lixin Ma, Tai Li, Lingping Kong, Milan He, Shichen Li, Xiaohui Zhao, Bize Yang, Linnan Chen, Wenqian Shi, Cen Yang, Lingshuang Wang, Yongli Li, Fanjiang Kong, Sijia Lu, Kun Kou |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Mol Breed |
| Popis: |
Soybean (Glycine max (L.) Merrill) is an important legume crop worldwide. Plant height (PH) is a quantitative trait that is closely related to node number (NN) and internode length (IL) on the main stem, which together affect soybean yield. To identify candidate genes controlling these three traits in soybean, we examined a recombinant inbred line (RIL) population derived from a cross between two soybean varieties with semi-determinate stems (Dt1Dt1Dt2Dt2), JKK378 and HXW. A quantitative trait locus (QTL) named qPH18 was identified that simultaneously controls PH, NN, and IL; this region harbors the semi-determinant gene Dt2. Sequencing of the Dt2 promoter from JKK378 identified three polymorphisms relative to HXW, including two single nucleotide polymorphism (SNPs) and an 18-bp insertion/deletion polymorphism (Indel). Dt2 expression was lower in the qPH18(JKK378) group than in the qPH18(HXW) group, whereas the expression level of the downstream gene Dt1 showed the opposite tendency. A transient transfection assay confirmed that Dt2 promoter activity is lower in JKK378 compared to HXW. We propose that the polymorphisms in the dominant Dt2 promoter underlie the differences in Dt2 expression and its downstream gene Dt1 in the two parents, thereby affecting PH, NN, IL, and grain weight per plant without altering stem growth habit. Compared to the PH18(HXW) allele, the qPH18(JKK378) allele suppresses Dt2 expression, which releases the inhibition of Dt1 expression, thus enhancing NN and grain yield. Our findings shed light on the mechanism underlying NN and PH in soybean and provide a molecular marker to facilitate breeding. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11032-021-01235-y. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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