Changes in expression of neuropeptides and their receptors in the hypothalamus and gastrointestinal tract of calorie restricted hens
| Autor: | Andrea Tamas, Zoltán Szilvássy, István Komlósi, János Oláh, József Németh, Ádám Simon, Dóra Reglődi, András Jávor, Levente Czeglédi |
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| Rok vydání: | 2017 |
| Předmět: |
Receptors
Neuropeptide endocrine system medicine.medical_specialty Calorie restriction Hypothalamus Neuropeptide 030209 endocrinology & metabolism Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Internal medicine Gene expression medicine Animals Agrártudományok Tissue Distribution Galanin Receptor Caloric Restriction General Environmental Science Neuropeptides digestive oral and skin physiology Proventriculus Jejunum Endocrinology Gene Expression Regulation Állattenyésztési tudományok Neurology Organ Specificity Female Chickens hormones hormone substitutes and hormone antagonists 030217 neurology & neurosurgery Neuromedin U |
| Zdroj: | Acta Biologica Hungarica. 68:237-247 |
| ISSN: | 1588-256X 0236-5383 |
| DOI: | 10.1556/018.68.2017.3.1 |
| Popis: | The list of orexigenic and anorexigenic peptides, those are known to alter feed intake, is continuously growing. However, most of them are studied in mammalian species. We aimed to investigate plasma level and mRNA expression of the pituitary adenylate cyclase-activating polypeptide (PACAP), gene expression of its receptor (PAC1), furthermore the gene expression of galanin (GAL), neuromedin U (NMU), and its two receptors (NMUR1 and NMUR2) in the hypothalamus, proventriculus, and jejunum of hens exposed to 40% calorie restriction. Feed restriction resulted in a 88% increase in mRNA and a 27% increase in peptide level of PACAP in proventriculus measured with qPCR and RIA, respectively. Increases were found in the gene expression of PAC1 (49%) and NMUR1 (63%) in the hypothalamus. Higher expressions of peptide encoding genes (76% for PACAP, 41% for NMU, 301% for NMUR1 and 308% for GAL, P < 0.05) were recorded in the jejunum of hens exposed to restricted nutrition. The results indicate that PACAP level responds to calorie restriction in the proventriculus and jejunum, but not in the hypothalamus and plasma. |
| Databáze: | OpenAIRE |
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