Modification of cell cycle and viability of TLX5 lymphoma in vitro by sulfoxide-ruthenium compounds and cisplatin detected by flow cytometry
| Autor: | Giovanni Salerno, Gianni Sava, Moreno Cocchietto, Alberta Bergamo, Ilaria Capozzi, K. Clerici |
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| Přispěvatelé: | Capozzi, I, Clerici, K, Cocchietto, M, Salerno, G, Bergamo, A, Sava, G |
| Rok vydání: | 1998 |
| Předmět: |
Lung Neoplasms
Lymphoma Cell Survival Antineoplastic Agents In Vitro Techniques Toxicology Ruthenium Flow cytometry Mice chemistry.chemical_compound Organometallic Compounds Tumor Cells Cultured medicine Animals Dimethyl Sulfoxide Propidium iodide Cytotoxicity Cisplatin medicine.diagnostic_test Cell Cycle Acridine orange DNA Neoplasm General Medicine Cell cycle Flow Cytometry Molecular biology In vitro Bromodeoxyuridine Biochemistry chemistry Doxorubicin Mice Inbred CBA DNA fragmentation medicine.drug |
| Zdroj: | Chemico-Biological Interactions. 113:51-64 |
| ISSN: | 0009-2797 |
| DOI: | 10.1016/s0009-2797(98)00022-2 |
| Popis: | The effects of Na[trans-RuCl4(DMSO)Im] (NAMI), Na[trans-RuCl4(TMSO)Ind] (TIND) and Na[trans-RuCl4(TMSO)Iq] TEQU) were tested in vitro on TLX5 lymphoma cells in comparison to cisplatin by means of the sulforhodamine-B test SRB) for protein content determination, by acridine orange and propidium iodide staining and by means of the bromodeoxyuridine test, for cell cycle modifications. After l h drug exposure with metal-based drugs, TLX5 lymphoma cells require a further 72 h in vitro cultivation to show alteration of cell cycle. Ruthenium compounds show a different pattern of effects: TEQU causes the same dose-dependent cytotoxicity and DNA fragmentation shown by cisplatin, TIND reduces absorbance with the SRB test and slightly increases S and G2M populations with a time-dependent drug exposure of tumour cells, and NAMI is virtually devoid of any detectable effect. By in vivo bioassay of in vitro treated tumour cells, TIND and TEQU are effective independently of the time of drug exposure of tumour cells, this effect being confirmed by the same cell uptake of ruthenium after l or 4 h treatment, determined by atomic absorption spectroscopy. These data stress the lack of the involvement of direct cytotoxic effects in the potent anti-metastatic action of NAMI. |
| Databáze: | OpenAIRE |
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