A panel of human neutralizing mAbs targeting SARS-CoV-2 spike at multiple epitopes
| Autor: | Adi Bercovich-Kinori, Hadas Tamir, Ayelet Zauberman, Efi Makdasi, Eyal Epstein, Osnat Rosen, Adva Mechaly, Shay Weiss, Theodor Chitlaru, Hagit Achdout, Tomer Israely, Tal Noy-Porat, Yfat Yahalom-Ronen, Nir Paran, Yinon Levy, Ronit Rosenfeld, Shmuel C. Shapira, Shmuel Yitzhaki, Sharon Melamed, Ohad Mazor, Ron Alcalay, Ma'ayan Israeli, Eldar Peretz |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Phage display medicine.drug_class medicine.medical_treatment Science viruses General Physics and Astronomy 02 engineering and technology Peptidyl-Dipeptidase A Monoclonal antibody Antibodies Viral Epitope Virus General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences Betacoronavirus Epitopes Peptide Library Chlorocebus aethiops medicine Animals Humans Protein Interaction Domains and Motifs Peptide library lcsh:Science Vero Cells Multidisciplinary biology SARS-CoV-2 Antibodies Monoclonal Immunotherapy General Chemistry 021001 nanoscience & nanotechnology Virology Antibodies Neutralizing 030104 developmental biology Epitope mapping Viral infection Spike Glycoprotein Coronavirus biology.protein lcsh:Q Angiotensin-Converting Enzyme 2 Antibody 0210 nano-technology Epitope Mapping Protein Binding |
| Zdroj: | Nature Communications Nature Communications, Vol 11, Iss 1, Pp 1-7 (2020) |
| ISSN: | 2041-1723 |
| Popis: | The novel highly transmissible human coronavirus SARS-CoV-2 is the causative agent of the COVID-19 pandemic. Thus far, there is no approved therapeutic drug specifically targeting this emerging virus. Here we report the isolation and characterization of a panel of human neutralizing monoclonal antibodies targeting the SARS-CoV-2 receptor binding domain (RBD). These antibodies were selected from a phage display library constructed using peripheral circulatory lymphocytes collected from patients at the acute phase of the disease. These neutralizing antibodies are shown to recognize distinct epitopes on the viral spike RBD. A subset of the antibodies exert their inhibitory activity by abrogating binding of the RBD to the human ACE2 receptor. The human monoclonal antibodies described here represent a promising basis for the design of efficient combined post-exposure therapy for SARS-CoV-2 infection. Here, Noy-Porat, Makdasi et al. report the isolation of a panel of neutralizing mAbs selected against SARS-CoV-2 receptor-binding domain (RBD) from a phage display library constructed based on patient samples collected in the acute phase of the disease, which show efficient neutralizing activities against authentic virus in vitro. |
| Databáze: | OpenAIRE |
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