Loss of Snord116 alters cortical neuronal activity in mice: a preclinical investigation of Prader-Willi syndrome
| Autor: | Matteo Cerri, Andrea Freschi, Blanco María Encarnación, Mojtaba Bandarabadi, Andrea Armirotti, Marta Pace, Antoine Roger Adamantidis, Michela Chiappalone, Ilaria Colombi, Matteo Falappa, Valter Tucci, Roberto Amici |
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| Přispěvatelé: | Pace M., Colombi I., Falappa M., Freschi A., Bandarabadi M., Armirotti A., Encarnacion B.M., Adamantidis A.R., Amici R., Cerri M., Chiappalone M., Tucci V. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Sleep REM Sleep spindle 610 Medicine & health Biology Electroencephalography 03 medical and health sciences Genomic Imprinting Mice 0302 clinical medicine Neurodevelopmental disorder Genetics medicine Premovement neuronal activity Animals Humans RNA Small Nucleolar Molecular Biology Genetics (clinical) Neurons medicine.diagnostic_test Eye movement General Medicine medicine.disease Sleep in non-human animals electroencephalography genes neurons prader-willi syndrome rem sleep mice sleep sleep spindles Disease Models Animal 030104 developmental biology Phenotype Wakefulness Genomic imprinting Sleep Neuroscience Prader-Willi Syndrome 030217 neurology & neurosurgery |
| Zdroj: | Pace, Marta; Colombi, Ilaria; Falappa, Matteo; Freschi, Andrea; Bandarabadi, Mojtaba; Armirotti, Andrea; Encarnación, Blanco María; Adamantidis, Antoine R; Amici, Roberto; Cerri, Matteo; Chiappalone, Michela; Tucci, Valter (2020). Loss of Snord116 alters cortical neuronal activity in mice: a preclinical investigation of Prader-Willi syndrome. Human molecular genetics, 29(12), pp. 2051-2064. Oxford University Press 10.1093/hmg/ddaa084 |
| Popis: | Prader–Willi syndrome (PWS) is a neurodevelopmental disorder that is characterized by metabolic alteration and sleep abnormalities mostly related to rapid eye movement (REM) sleep disturbances. The disease is caused by genomic imprinting defects that are inherited through the paternal line. Among the genes located in the PWS region on chromosome 15 (15q11-q13), small nucleolar RNA 116 (Snord116) has been previously associated with intrusions of REM sleep into wakefulness in humans and mice. Here, we further explore sleep regulation of PWS by reporting a study with PWScrm+/p− mouse line, which carries a paternal deletion of Snord116. We focused our study on both macrostructural electrophysiological components of sleep, distributed among REMs and nonrapid eye movements. Of note, here, we study a novel electroencephalography (EEG) graphoelements of sleep for mouse studies, the well-known spindles. EEG biomarkers are often linked to the functional properties of cortical neurons and can be instrumental in translational studies. Thus, to better understand specific properties, we isolated and characterized the intrinsic activity of cortical neurons using in vitro microelectrode array. Our results confirm that the loss of Snord116 gene in mice influences specific properties of REM sleep, such as theta rhythms and, for the first time, the organization of REM episodes throughout sleep–wake cycles. Moreover, the analysis of sleep spindles present novel specific phenotype in PWS mice, indicating that a new catalog of sleep biomarkers can be informative in preclinical studies of PWS. |
| Databáze: | OpenAIRE |
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