Assessment of anti-inflammatory, antinociceptive, immunomodulatory, and antioxidant activities ofCajanus cajanL. seeds cultivated in Egypt and its phytochemical composition
| Autor: | SM Mohamed, Azza A. Matloub, Mona E Aboutabl, Nabaweya A. Ibrahim, Emad M. Hassan |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Male
0301 basic medicine Time Factors Antioxidant DPPH Butanols medicine.medical_treatment Anti-Inflammatory Agents Pharmaceutical Science Carrageenan Antioxidants Nociceptive Pain Lipid peroxidation Mice chemistry.chemical_compound Cajanus Drug Discovery Analgesics Behavior Animal Traditional medicine biology Chemistry General Medicine Biphenyl compound Biochemistry Seeds Cytokines Molecular Medicine Egypt Female Inflammation Mediators medicine.drug_class Gas Chromatography-Mass Spectrometry Anti-inflammatory 03 medical and health sciences Phytol Picrates medicine Animals Hexanes Immunologic Factors Inflammation Pharmacology Plants Medicinal Plant Extracts Biphenyl Compounds biology.organism_classification Rats Disease Models Animal 030104 developmental biology Complementary and alternative medicine Unsaponifiable Solvents Lipid Peroxidation Phytotherapy |
| Zdroj: | Europe PubMed Central |
| ISSN: | 1744-5116 1388-0209 |
| DOI: | 10.3109/13880209.2015.1078383 |
| Popis: | Cajanus cajan L. (Fabaceae), a food crop, is widely used in traditional medicine.The phytochemical composition of C. cajan seeds and evaluation of the anti-inflammatory, immunomodulatory, antinociceptive, and antioxidant activities were studied.Unsaponifiable matter and fatty acids were analyzed by GC and GC/MS. The n-butanol fraction was chromatographed on polyamide column. The anti-inflammatory activity of hexane extract (200 and 400 mg/kg, p.o.) was evaluated using the carrageenan-induced rat paw edema at 1, 2, and 3 h. The serum tumor necrosis factor-α, interleukin-6, and immunoglobulin G levels were detected by ELISA. The hexane extract antinociceptive activity was determined by adopting the writhing test in mice. DPPH radical scavenging, total reduction capability, and inhibition of lipid peroxidation of butanol fraction were evaluated.Twenty-one unsaponifiable compounds (mainly phytol, 2,6-di-(t-butyl)-4-hydroxy-4-methyl-2,5-cyclohexadiene-1-one, β-sitosterol, stigmasterol, and campesterol), as well as 12 fatty acids (primarily 9,12-octadecadienoic and palmitic acids) were identified in hexane extract of C. cajan seeds. n-BuOH fraction contains quercetin-3-O-β-d-glucopyranoside, orientin, vitexin, quercetin, luteolin, apigenin, and isorhamnetin. For the first time, quercetin-3-O-β-d-glucopyranoside is isolated from C. cajan plant. The hexane extract (200 and 400 mg/kg) inhibited carrageenan-induced inflammation by 85 and 95%, respectively, 3 h post-carrageenan challenge. This was accompanied by an 11 and 20%, 8 and 13%, respectively, decrease of TNF-α and IL-6, as well as significant decrease in IgG serum levels. Moreover, hexane extract (200 and 400 mg/kg) decreased the number of writhings by 61 and 83%, respectively. The butanol fraction showed DPPH radical scavenging (inhibitory concentration (IC50) value: 9.07 μg/ml). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|