Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive

Autor: Edson Crusca Junior, Hanyeny Raiely Leite Silva, Jessica M A Blair, Wagner Costa Macedo, Nilce Maria Soares, Cesar Augusto Roque-Borda, Marlus Chorilli, Eduardo Vicente, Elisabete Aparecida Lopes Guastalli, Andréia Bagliotti Meneguin, Jéssica Aparecida Serafim, Zoe Pikramenou, Silvio Rainho Teixeira
Přispěvatelé: Universidade Estadual Paulista (Unesp), Biological Institute, University of Birmingham
Rok vydání: 2021
Předmět:
Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 0141-8130
Popis: Made available in DSpace on 2021-06-25T11:00:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-07-31 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Microencapsulation is a potential biotechnological tool, which can overcome antimicrobial peptides (AMP) instabilities and reduce toxic side effects. Thus, this study evaluates the antibacterial activities of the Ctx(Ile21)-Ha AMP against multidrug-resistant (MDR) and non-resistant bacteria and develop and characterize peptide-loaded microparticles coated with the enteric polymers hydroxypropylmethylcellulose acetate succinate (HPMCAS) and hydroxypropylmethylcellulose phthalate (HPMCP). Ctx(Ile21)-Ha was obtained by solid phase peptide synthesis (SPPS) method, purified and characterized by HPLC and Mass Spectrometry. The peptide exhibited potent antibiotic activities against Salmonella enteritidis, Salmonella typhimurium, Pseudomonas aeruginosa (MDR), Acinetobacter baumannii (MDR), and Staphylococcus aureus (MDR). Ctx(Ile21)-Ha microencapsulation was performed by ionic gelation with high efficiency, maintaining the physical-chemical stability. Ctx(Ile21)-Ha coated-microparticles were characterized by DSC, TGA, FTIR-Raman, XRD and SEM. Hemolytic activity assay demonstrated that hemolysis was decreased up to 95% compared to single molecule. In addition, in vitro release control profile simulating different portions of gastrointestinal tract was performed and showed the microcapsules' ability to protect the peptide and release it in the intestine, aiming pathogen's location, mainly by Salmonella sp. Therefore, use of microencapsulated Ctx(Ile21)-Ha can be allowed as an antimicrobial controller in monogastric animal production as an oral feed additive (antimicrobial controller), being a valuable option for molecules with low therapeutic indexes or high hemolytic rates. São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, Jaboticabal São Paulo State University (Unesp) Institute of Chemistry, Araraquara São Paulo State University (Unesp) School of Sciences and Engineering, Tupã São Paulo State University (Unesp) School of Pharmaceutical Sciences, Araraquara São Paulo State University (Unesp) School of Technology and Sciences, Presidente Prudente Poultry Health Specialized Laboratory Biological Institute, Bastos Institute of Microbiology and Infection University of Birmingham School of Chemistry University of Birmingham São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, Jaboticabal São Paulo State University (Unesp) Institute of Chemistry, Araraquara São Paulo State University (Unesp) School of Sciences and Engineering, Tupã São Paulo State University (Unesp) School of Pharmaceutical Sciences, Araraquara São Paulo State University (Unesp) School of Technology and Sciences, Presidente Prudente FAPESP: 2016/00446-7
Databáze: OpenAIRE
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