Full protection against African horsesickness (AHS) in horses induced by baculovirus-derived AHS virus serotype 4 VP2, VP5 and VP7
| Autor: | Jorge L. Martínez-Torrecuadrada, M. Diaz-Laviada, C. Vela, Carmen Navarro Sánchez, Polly Roy, J I Casal, José Manuel Sánchez-Vizcaíno |
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| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: |
Serotype
Antigenicity viruses Enzyme-Linked Immunosorbent Assay Spodoptera Biology Antibodies Viral Virus law.invention Capsid Antigen Affinity chromatography Neutralization Tests law African Horse Sickness Virology Chlorocebus aethiops African Horse Sickness Virus Animals Horses Antigens Viral Vero Cells Vaccines Synthetic Expression vector virus diseases Viral Vaccines biochemical phenomena metabolism and nutrition Recombinant DNA biology.protein Antibody Baculoviridae |
| Zdroj: | Repositorio de Resultados de Investigación del INIA INIA: Repositorio de Resultados de Investigación del INIA Scopus-Elsevier Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA |
| Popis: | African horsesickness virus serotype 4 (AHSV-4) outer capsid protein VP2, or VP2 and VP5 plus inner capsid protein VP7, derived from single or dual recombinant baculovirus expression vectors were used in different combinations to immunize horses. When the proteins were purified by affinity chromatography, the combination of all three proteins induced low levels of neutralizing antibodies and conferred protection against virulent virus challenge. However, purified VP2 or VP2 and VP5 in the absence of VP7 failed to induce neutralizing antibodies and protection. Immunization with non-purified proteins enhanced the titres of neutralizing antibodies. Again, the combination of the three proteins was able to confer total protection to immunized horses, which showed absence of viraemia. The antigenicity of recombinant VP2 was analysed with a collection of 30 MAbs. Both purified and unpurified recombinant VP2 proteins showed different antigenic patterns in comparison to that of VP2 on virions. An immunization experiment with four more horses confirmed these results. The vaccine described here would not only prevent the disease, but would drastically reduce the propagation of the virus by vectors. |
| Databáze: | OpenAIRE |
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