T cell lines generated with type II collagen proliferate in an autologous mixed lymphocyte response
| Autor: | Brian Catchpole, Norman A. Staines, A.S. Hamblin |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Protein Denaturation
Hot Temperature Ovalbumin T cell T-Lymphocytes Immunology Population Antigen presentation Molecular Sequence Data Antigen-Presenting Cells Epitopes T-Lymphocyte Lymphocyte Activation Epitope Cell Line Immunophenotyping Immune system medicine Immunology and Allergy Animals Amino Acid Sequence education Antigen-presenting cell Collagen Type II education.field_of_study business.industry T lymphocyte Molecular biology Coculture Techniques Peptide Fragments Rats Kinetics medicine.anatomical_structure Cell culture Cattle Lymphocyte Culture Test Mixed business |
| Zdroj: | Journal of autoimmunity. 17(3) |
| ISSN: | 0896-8411 |
| Popis: | Collagen-induced arthritis (CIA) is a T cell-dependent disease induced in susceptible rodents by immunizing with bovine type II collagen (bCII). In order to study T cell responses, a programme to generate bCII-specific T cell lines from arthritic rats was initiated. Lymph node cells from bCII-immune WA/KIR/kcl rats were cultured with bCII in vitro, and the T cells were isolated and restimulated with bCII-pulsed antigen presenting cells (APC) (thymus cells or splenic low density cells). However, T cells, generated initially to bCII, subsequently proliferated upon co-culture with syngeneic APC even in the absence of bCII. This suggests that exposure to bCII resulted in the activation of a population of self-reactive T cells which proliferate in an autologous mixed lymphocyte response. In contrast, short-term T cell lines generated to ovalbumin, heat-denatured bCII and the collagen peptide bCII(184-198) proliferated in response to specific antigen-pulsed APC without demonstrating self-reactivity. Since denatured bCII and bCII(184-198) peptide are not arthritogenic and failed to generate self reactivity in vitro, this suggests that the native triple helical conformation of bCII is required for stimulating autoreactive T cell responses. |
| Databáze: | OpenAIRE |
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