Casein kinase 2-interacting protein-1, an inflammatory signaling molecule interferes with TNF reverse signaling in human model cells
| Autor: | Kata Juhasz, Erno Duda, Zsolt Balogi, Alois Sonnleitner, Anna M. Lipp, Ágnes Zvara, Benedikt Nimmervoll |
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| Rok vydání: | 2013 |
| Předmět: |
Lipopolysaccharides
Transcriptional Activation Immunology Apoptosis Inflammation Monocytes medicine Humans Immunology and Allergy Transgenes Promoter Regions Genetic Feedback Physiological biology Tumor Necrosis Factor-alpha Monocyte HEK 293 cells Intracellular Signaling Peptides and Proteins Membrane Proteins Molecular biology Cell biology DC-SIGN Protein Transport HEK293 Cells medicine.anatomical_structure biology.protein Tumor necrosis factor alpha Inflammation Mediators medicine.symptom Casein kinase 2 Signal transduction Carrier Proteins Signal Transduction |
| Zdroj: | Immunology Letters. 152:55-64 |
| ISSN: | 0165-2478 |
| DOI: | 10.1016/j.imlet.2013.04.001 |
| Popis: | When transmembrane form of tumor necrosis factor (mTNF) interacts with its cognate receptors or agonistic antibodies signaling pathways are activated in the ligand expressing cells. This "reverse signaling" appears a fine-tuning control mechanism in the immune response. Despite a clinical relevance key molecules of TNF reverse signaling and their functions remain elusive. We examined the role of CKIP-1, an interacting partner of the N terminal fragment of mTNF in inflammation and TNF reverse signaling. We found that CKIP-1 expression was elevated upon LPS challenge in THP-1 human monocyte model cells. Overexpression of CKIP-1 triggered classical activation of THP-1 cells and transactivated the human TNF promoter when co-expressed with c-Jun in the HEK293 model system. TNF reverse signaling induced a massive translocation of CKIP-1 from the plasma membrane to intracellular compartments in THP-1 cells. Expression of the N terminal fragment of mTNF in HEK293 cells resembled the effects of TNF reverse signaling with respect to relocalization of CKIP-1. In parallel with the translocation, CKIP-1-triggered activation of THP-1 cells was antagonized by TNF reverse signaling. Similarly, the presence of the N terminal fragment of mTNF inhibited CKIP-1 mediated TNF promoter activation in HEK293 cells. Both TNF reverse signaling in THP-1 cells and expression of the N terminal fragment of mTNF in HEK293 cells were found to induce apoptosis that could be prevented by overexpression of CKIP-1. Our findings demonstrate that CKIP-1 activates pro-inflammatory pathways and interferes with TNF reverse signaling induced apoptosis in human model cells. |
| Databáze: | OpenAIRE |
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