Characterization of Mono- and Mixed-Culture Campylobacter jejuni Biofilms
| Autor: | Vildan Caner, Tuba Ica, Hung Duc Nguyen, Bulbul Ahmed, Haluk Beyenal, Douglas R. Call, Ozlem Istanbullu |
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| Rok vydání: | 2012 |
| Předmět: |
confocal microscopy
medicine.disease_cause Applied Microbiology and Biotechnology biofilm Different structure monoculture Environmental Microbiology Environmental conditions Bacteria (microorganisms) Live/dead staining Microscopy Confocal Microbial Viability Ecology biology Confocal laser scanning microscope Contaminated water Physiological state article methodology staining bacterium Pseudomonas aeruginosa Culturable gastroenteritis Biotechnology Fastidious organism microbial viability Flow rate survival Campylobacter jejuni Microbiology medicine Animals Animalia Microaerophile flow pattern growth development and aging Sharp contrast Bacteria Staining and Labeling Thermophile Biofilm biochemical phenomena metabolism and nutrition biology.organism_classification Oxygen Microaerophilic bacteria Biofilms physiology Environmental pathways Cell culture physiological response metabolism Food Science |
| Zdroj: | Applied and Environmental Microbiology. 78:1033-1038 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.07364-11 |
| Popis: | Campylobacter jejuni , one of the most common causes of human gastroenteritis, is a thermophilic and microaerophilic bacterium. These characteristics make it a fastidious organism, which limits its ability to survive outside animal hosts. Nevertheless, C. jejuni can be transmitted to both humans and animals via environmental pathways, especially through contaminated water. Biofilms may play a crucial role in the survival of the bacterium under unfavorable environmental conditions. The goal of this study was to investigate survival strategies of C. jejuni in mono- and mixed-culture biofilms. We grew monoculture biofilms of C. jejuni and mixed-culture biofilms of C. jejuni with Pseudomonas aeruginosa . We found that mono- and mixed-culture biofilms had significantly different structures and activities. Monoculture C. jejuni biofilms did not consume a measurable quantity of oxygen. Using a confocal laser scanning microscope (CLSM), we found that cells from monoculture biofilms were alive according to live/dead staining but that these cells were not culturable. In contrast, in mixed-culture biofilms, C. jejuni remained in a culturable physiological state. Monoculture C. jejuni biofilms could persist under lower flow rates (0.75 ml/min) but were unable to persist at higher flow rates (1 to 2.5 ml/min). In sharp contrast, mixed-culture biofilms were more robust and were unaffected by higher flow rates (2.5 ml/min). Our results indicate that biofilms provide an environmental refuge that is conducive to the survival of C. jejuni . |
| Databáze: | OpenAIRE |
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