Role of TrkA signaling during tadpole tail regeneration and early embryonic development inXenopus laevis
| Autor: | Eisuke Nishida, Akira Iimura, Morioh Kusakabe |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Tail
Mesoderm animal structures Carbazoles Xenopus Embryonic Development Nerve Tissue Proteins Receptors Nerve Growth Factor Tropomyosin receptor kinase B Tropomyosin receptor kinase A Biology Tropomyosin receptor kinase C Indole Alkaloids Xenopus laevis 03 medical and health sciences Nerve Growth Factor Notochord Genetics medicine Animals Regeneration Receptor trkC Receptor trkA 030304 developmental biology 0303 health sciences Gene Expression Regulation Developmental Azepines Cell Biology biology.organism_classification Cell biology medicine.anatomical_structure nervous system Neurula Larva Benzamides embryonic structures biology.protein Signal Transduction Neurotrophin |
| Zdroj: | Genes to Cells. 25:86-99 |
| ISSN: | 1365-2443 1356-9597 |
| DOI: | 10.1111/gtc.12740 |
| Popis: | Neurotrophic signaling regulates neural cell behaviors in development and physiology, although its role in regeneration has not been fully investigated. Here, we examined the role of neurotrophic signaling in Xenopus laevis tadpole tail regeneration. After the tadpole tails were amputated, the expression of neurotrophin ligand family genes, especially ngf and bdnf, was up-regulated as regeneration proceeded. Moreover, notochordal expression of the NGF receptor gene TrkA, but not that of other neurotrophin receptor genes TrkB and TrkC, became prominent in the regeneration bud, a structure arising from the tail stump after tail amputation. The regenerated tail length was significantly shortened by the pan-Trk inhibitor K252a or the TrkA inhibitor GW-441756, but not by the TrkB inhibitor ANA-12, suggesting that TrkA signaling is involved in elongation of regenerating tails. Furthermore, during Xenopus laevis embryonic development, TrkA expression was detected in the dorsal mesoderm at the gastrula stage and in the notochord at the neurula stage, and its knockdown led to gastrulation defects with subsequent shortening of the body axis length. These results suggest that Xenopus laevis TrkA signaling, which can act in the mesoderm/notochord, plays a key role in body axis elongation during embryogenesis as well as tail elongation during tadpole regeneration. |
| Databáze: | OpenAIRE |
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