Vitellogenin expression in the oilseed rape pest Meligethes aeneus

Autor: Michael Kristensen, Caroline Kaiser, Christina Lazaridou, Karl-Martin Vagn Jensen, Rodrigo Labouriau
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0106 biological sciences
0301 basic medicine
Male
egg yolk protein
medicine.disease_cause
01 natural sciences
Vitellogenins
chemistry.chemical_compound
Tubulin
Molecular marker
Coleoptera
Vitellogenins/metabolism
OVIPOSITION
Female
Pollen beetle
COLORADO POTATO BEETLE
Seasons
STRAIN
food.ingredient
animal structures
GENES
oilseed rape
Zoology
Brassica
Biology
General Biochemistry
Genetics and Molecular Biology
Coleoptera/metabolism
03 medical and health sciences
Vitellogenin
food
Yolk
Pollen
Botany
medicine
Animals
Ecology
Evolution
Behavior and Systematics
SEX DETERMINATION
Colorado potato beetle
qRT-PCR
Tubulin/metabolism
pollen beetle
biology.organism_classification
Brassicogethes aeneus
010602 entomology
030104 developmental biology
chemistry
Insect Science
biology.protein
gene expression
PEST analysis
Agronomy and Crop Science
RESISTANCE
Meligethes aeneus
Zdroj: Kaiser, C, Lazaridou, C, Jensen, K-M V, Labouriau, R & Kristensen, M 2019, ' Vitellogenin expression in the oilseed rape pest Meligethes aeneus ', Insect Science, vol. 26, no. 2, pp. 274-282 . https://doi.org/10.1111/1744-7917.12539
DOI: 10.1111/1744-7917.12539
Popis: When investigating insecticide resistance of pest insects, for example, the pollen beetle Meligethes aeneus, it is relevant to differentiate toxicological and molecular genetic data between male and female specimens. A molecular sex determination method would allow resistance testing to be run without prior sorting of the samples. A one-step quantitative RT-PCR method for quantification of the yolk protein vitellogenin expression in the pollen beetle was established. The expression level of vitellogenin relative to tubulin was determined. Pollen beetles were tested at different time points during their development to determine if vitellogenin is a reliable molecular marker for detection of sexually mature females. The differentiation between females and males by relative expression of vitellogenin to tubulin is conditional regarding the life cycle. Sexually mature females and males could easily be distinguished, whereas immature specimens could not be seperated. Vitellogenin expression is a successful marker for identification of sexually mature pollen beetles. Females from the spring populations showed vitellogenin expression when the temperature was above 10.2°C. Further, detailed observations of vitellogenin throughout the spring indicated a strong relationship between daily temperatures and vitellogenin expression, which is an indicator of oviposition ability.
Databáze: OpenAIRE
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