Linc02527 promoted autophagy in Intrahepatic cholestasis of pregnancy
| Autor: | Zhanyu Zhang, Xiaocui Zhong, Xiao-Ling Gan, Jianguo Hu, Lina Hu, Lei Zhang, Xiaojing Dong, Xiaodong Luo, Xinru Deng, Li Liu, Yangyang Gong, Tinghe Yu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Cyclin-Dependent Kinase Inhibitor p21
0301 basic medicine Cancer Research Placenta Immunology ATG5 Cholestasis Intrahepatic Autophagy-Related Protein 7 Autophagy-Related Protein 5 Cell Line Rats Sprague-Dawley Mice 03 medical and health sciences Cellular and Molecular Neuroscience Cholestasis Pregnancy Chloroquine Autophagy medicine Animals Humans Gene silencing Gene Silencing lcsh:QH573-671 Cell Proliferation integumentary system lcsh:Cytology Chemistry Cell Biology medicine.disease Mice Inbred C57BL Pregnancy Complications MicroRNAs 030104 developmental biology medicine.anatomical_structure Cell culture Models Animal Cancer research Female RNA Long Noncoding Y-Box-Binding Protein 1 Biomarkers Cholestasis of pregnancy medicine.drug |
| Zdroj: | Cell Death and Disease, Vol 9, Iss 10, Pp 1-14 (2018) |
| ISSN: | 2041-4889 |
| DOI: | 10.1038/s41419-018-1013-z |
| Popis: | LncRNA plays a crucial role in human disease. However, the expression and function of LncRNA in ICP(Intrahepatic cholestasis of pregnancy) is still not fully elucidated. In this study, we found Linc02527 was increased expression in placenta and serum of ICP patients. Ectopically expression of Linc02527 promoted autophagy and proliferate in HTR8 cells. Silencing Linc02527 suppressed the autophagy and proliferate in HTR8 cells. Mechanically study revealed that Linc02527 regulated the expression of ATG5 and ATG7 by sponging miR-3185. Linc02527 directly binding to YBX1 and activated P21. The growth of C57 mouse was retarded when autophagy was activated. In normal condition, inhibited autophagy using chloroquine did not affect the growth of C57 mouse. However, in the condition of autophagy was activated, inhibited autophagy using chloroquine can improve the growth of C57 mouse. Overall, the results of this study identified Linc02527 as a candidate biomarker in ICP and a potential target for ICP therapy. Chloroquine was a potential drug for ICP therapy. |
| Databáze: | OpenAIRE |
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