Comprehensive N-glycosylation analysis of immunoglobulin G from dried blood spots
| Autor: | Ana Momčilović, Ivan Gudelj, Gordan Lauc, Jelena Šimunović, Irena Trbojević-Akmačić, Frano Vučković, Marija Pezer, Julija Jurić, Natali Nakić, Marija Vilaj |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
Glycan Glycosylation Chromatography Spots biology IgG glycome biomarker dried blood spots high-throughput ultra-performance liquid chromatography Chemistry Biological age 010401 analytical chemistry 01 natural sciences Biochemistry Glycome Immunoglobulin G 0104 chemical sciences 03 medical and health sciences N-linked glycosylation biology.protein Humans Dried Blood Spot Testing Dried blood Chromatography High Pressure Liquid 030304 developmental biology Blood sampling |
| Zdroj: | Glycobiology |
| ISSN: | 1460-2423 |
| DOI: | 10.1093/glycob/cwz061 |
| Popis: | Immunoglobulin G (IgG) glycans are emerging as a new putative biomarker for biological age and different diseases, requiring a robust workflow for IgG glycome analysis, ideally beginning with a simple and undemanding sampling procedure. Here, we report the first comprehensive study on total N-glycans of IgG isolated from dried blood spots (DBSs), which was performed in a high-throughput mode. We compared the IgG N-glycan profiles originating from DBS with those originating from plasma, compared different media for DBS collection, evaluated analytical variation and assessed IgG N-glycan profile stability for different storage conditions. In conclusion, we show that DBSs are a good and stable source material for a robust IgG N-glycan analysis by ultra-performance liquid chromatography, suitable for blood sampling in conditions where no trained personnel and necessary laboratory equipment are available. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|