Expression of Ciona intestinalis Variable Region-Containing Chitin-Binding Proteins during Development of the Gastrointestinal Tract and Their Role in Host-Microbe Interactions
| Autor: | Larry J. Dishaw, Assunta Liberti, Lenina Natale, Rosaria De Santis, Ivana Zucchetti, Maria Rosaria Pinto, Daniela Melillo, Gary W. Litman |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Pathology
Embryology Sea Squirts Organogenesis Immunofluorescence lcsh:Medicine Gene Expression Chitin Cell Fate Determination Chitin binding Animal Cells Molecular Cell Biology Morphogenesis Homeostasis Pattern Formation lcsh:Science Immune Response Cephalochordate Gastrointestinal tract Innate Immune System Multidisciplinary biology Microbiota Stomach Gene Expression Regulation Developmental Animal Models Cell biology Ciona intestinalis medicine.anatomical_structure Real-time polymerase chain reaction Host-Pathogen Interactions Endoderm Anatomy Cellular Types Immunohistochemical Analysis Research Article medicine.medical_specialty Histology Evolutionary Immunology Immunology In situ hybridization Research and Analysis Methods Immunomodulation Model Organisms Bacillus cereus medicine Genetics Escherichia coli Animals Immunoassays Immunohistochemistry Techniques Evolutionary Biology Blood Cells Metamorphosis Evolutionary Developmental Biology lcsh:R VCBPs Immunity Organisms Biology and Life Sciences Proteins Cell Biology biology.organism_classification Protein Structure Tertiary Ciona Histochemistry and Cytochemistry Techniques Gastrointestinal Tract host-microbe Immune System Immunologic Techniques lcsh:Q Clinical Immunology Organism Development Developmental Biology |
| Zdroj: | PLoS ONE PloS one 9 (2014). doi:10.1371/journal.pone.0094984 info:cnr-pdr/source/autori:Liberti, Assunta; Melillo, Daniela; Zucchetti, Ivana; Natale, Lenina; Dishaw, Larry J.; Litman, Gary W.; De Santis, Rosaria; Pinto, Maria Rosaria/titolo:Expression of Ciona intestinalis Variable Region-Containing Chitin-Binding Proteins during Development of the Gastrointestinal Tract and Their Role in Host-Microbe Interactions/doi:10.1371%2Fjournal.pone.0094984/rivista:PloS one/anno:2014/pagina_da:/pagina_a:/intervallo_pagine:/volume:9 PLoS ONE, Vol 9, Iss 5, p e94984 (2014) |
| ISSN: | 1932-6203 |
| Popis: | Variable region-containing chitin-binding proteins (VCBPs) are secreted, immune-type molecules that have been described in both amphioxus, a cephalochordate, and sea squirt, Ciona intestinalis, a urochordate. In adult Ciona, VCBP-A, -B and -C are expressed in hemocytes and the cells of the gastrointestinal tract. VCBP-C binds bacteria in the stomach lumen and functions as an opsonin in vitro. In the present paper the expression of VCBPs has been characterized during development using in situ hybridization, immunohistochemical staining and quantitative polymerase chain reaction (qPCR) technologies. The expression of VCBP-A and -C is detected first in discrete areas of larva endoderm and becomes progressively localized during differentiation in the stomach and intestine, marking the development of gut tracts. In "small adults" (1-2 cm juveniles) expression of VCBP-C persists and VCBP-A gradually diminishes, ultimately replaced by expression of VCBP-B. The expression of VCBP-A and -C in stage 7-8 juveniles, at which point animals have already started feeding, is influenced significantly by challenge with either Gram-positive or -negative bacteria. A potential role for VCBPs in gut-microbiota interactions and homeostasis is indicated. |
| Databáze: | OpenAIRE |
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