Fluoride effects on cell viability and ENaC expression in kidney epithelial cells
| Autor: | Cintia Kazuko Tokuhara, Mariana R. Santesso, Rodrigo Cardoso de Oliveira, Ligia S. Antonio, Flávia Amadeu de Oliveira, Gabriela Silva Neubern de Oliveira, Flávia Mauad Levy, Marília Afonso Rabelo Buzalaf |
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| Rok vydání: | 2021 |
| Předmět: |
Proteomics
Epithelial sodium channel 2019-20 coronavirus outbreak Cell Survival Health Toxicology and Mutagenesis Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) 010501 environmental sciences Kidney Toxicology 01 natural sciences Fluorides 03 medical and health sciences chemistry.chemical_compound medicine Viability assay 0105 earth and related environmental sciences 0303 health sciences Chemistry 030302 biochemistry & molecular biology Epithelial Cells TRANSPORTE EPITELIAL Cell biology medicine.anatomical_structure Cell culture Apoptosis sense organs Fluoride |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Fluoride (F) at micromolar (µM) concentrations induces apoptosis in several cell lines. Moreover, proteomic studies have shown major changes in the profile of proteins involved in signal transduction. These effects may negatively affect ion transport in the kidneys. The activity of epithelial sodium channels (ENaCs) is a limiting factor for sodium and water resorption in the kidneys, which is essential for the maintenance of the electrolyte balance and homeostasis of the body. Here we investigated the effects of F, at different concentrations (10, 40, 100, 200, and 400 μM), on the viability of renal epithelial cells (M-1), and ENaC expression. We showed that sodium fluoride (NaF) reduces cell viability in a concentration-dependent manner ( |
| Databáze: | OpenAIRE |
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