LncRNA RP11-307C12.11 promotes the growth of hepatocellular carcinoma by acting as a molecular sponge of miR-138
| Autor: | Yi-Quan Jiang, Yiming Huang, Haibo Li, Tong Zhang, Kaining Zeng, Yusheng Cheng, Tingting Xia, Yang Yang, Yinan Deng |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
congenital hereditary and neonatal diseases and abnormalities Gene knockdown Hepatology Oncogene Gastroenterology Biology medicine.disease digestive system diseases eye diseases 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Cyclin D1 Hepatocellular carcinoma mental disorders microRNA Cancer research medicine lcsh:Diseases of the digestive system. Gastroenterology 030211 gastroenterology & hepatology MTT assay Luciferase lcsh:RC799-869 miR-138 |
| Zdroj: | Liver Research, Vol 3, Iss 3, Pp 240-249 (2019) |
| ISSN: | 2542-5684 |
| Popis: | Background: Abnormal expression of long non-coding RNAs (lncRNAs) has been found in almost all tumors in humans, providing numerous potential diagnostic and prognostic biomarkers, and therapeutic targets. Materials and methods: The Cancer Genome Atlas (TCGA) database was used to screen potential LncRNAs, and 30 paired hepatocellular carcinoma (HCC) tissues were used to investigate RP11-307C12.11 expression levels by qRT-PCR and another 105 HCC tissues by in situ hybridizsation (ISH). RP11-307C12.11 overexpression and knockdown experiments were performed to investigate the effects of RP11-307C12.11 on HCC growth through in vitro and in vivo assays (MTT assay, colony formation assay, EdU assay, and xenograft model). The molecular mechanism underlying these effects was confirmed by MS2-RIP-assay, RIP assay, luciferase assay, and rescue experiments. Results: RP11-307C12.11 expression level was significantly higher in tumor tissues than in the adjacent normal tissues. Elevated RP11-307C12.11 expression level was associated with poor prognosis of HCC patients, and it may be represented as an independent prognostic biomarker in patients with HCC. Functionally, RP11-307C12.11 overexpression promoted HCC growth both in vitro and in vivo; however, its knockdown reversed these effects. Mechanistically, we found that RP11-307C12.11 expressed predominantly in the cytoplasm and sponged microRNA (miR)-138 to regulate its common target CCND1 and PDK1. Conclusions: Thus, we found that RP11-307C12.11 acts as an oncogene in HCC by binding to miR-138, which might provide a novel target for HCC therapy. Keywords: Long non-coding RNAs (lncRNAs), LncRNA RP11-307C12.11, Hepatocellular carcinoma (HCC), Growth, MicroRNA (MiR)-138 |
| Databáze: | OpenAIRE |
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