What the human sperm methylome tells us
| Autor: | Albert Salas-Huetos, Riccardo Aiese Cigliano, Cristina Camprubí, Nicolás Garrido, Joan Blanco |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male Cancer Research Piwi-interacting RNA Embryonic Development Semen Biology Epigenesis Genetic 03 medical and health sciences Genetics Humans Promoter Regions Genetic Spermatogenesis Gene Chromosomes Human X Chromosomes Human Y Methylation DNA Methylation Sperm Spermatozoa 030104 developmental biology CpG site DNA methylation CpG Islands |
| Zdroj: | Epigenomics. 9(10) |
| ISSN: | 1750-192X |
| Popis: | Aim: To characterize the sperm methylome in semen samples from 19 donors with proven fertility. Materials & methods: Bisulfite-converted sperm DNA was hybridized on the HumanMethylation450 Infinium BeadChip platform. CpG fluorescence intensities were extracted and converted to β-values. Results: The sperm methylome is highly homogeneous and hypomethylated. Genes with hypomethylated promoters are ontologically associated to biological functions related to spermatogenesis and embryogenesis. Sex chromosomes are the most hypomethylated chromosomes, supporting data that indicated their essential role in spermatogenesis. A total of 94 genes are resistant to demethylation, being strong candidates for transgenerational inheritance. Conclusion: Spermatozoa carry a homogeneous methylation profile that is a footprint of past events (spermatogenesis), is designed to facilitate future events (embryogenesis) and has a possible influence in the adult life (transgenerational effects). |
| Databáze: | OpenAIRE |
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