Morphologically and physiologically diverse fruits of two Lepidium species differ in allocation of glucosinolates into immature and mature seed and pericarp

Autor: Caroline Müller, Said Mohammed, Klaus Mummenhoff, Katharina Klupsch, Matthias Alexander Gesing, Guenter Theissen, Samik Bhattacharya
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Fruit and Seed Anatomy
Indoles
Physiology
Plant Science
Plant Reproduction
Lepidium
Genetically Modified Plants
chemistry.chemical_compound
Pericarp
Heterocyclic Compounds
Seed Germination
Morphogenesis
Chromatography
High Pressure Liquid
biology
Organic Compounds
Plant Anatomy
Genetically Modified Organisms
Eukaryota
food and beverages
General Medicine
Plants
Plant Dormancy
Chemistry
Plant Physiology
Seeds
Physical Sciences
Engineering and Technology
Medicine
Composition (visual arts)
Genetic Engineering
General Agricultural and Biological Sciences
Research Article
Biotechnology
Science
Glucosinolates
Germination
Bioengineering
Genetics and Molecular Biology
Fruits
Botany
Transgenic lines
Plant Defenses
Ecosystem
Diaspore (botany)
Organic Chemistry
Organisms
Chemical Compounds
Water
Biology and Life Sciences
Brassicaceae
biology.organism_classification
chemistry
Fruit
Glucosinolate
General Biochemistry
Dormancy
Biological dispersal
Plant Biotechnology
Developmental Biology
Zdroj: PLoS ONE, Vol 15, Iss 8, p e0227528 (2020)
PLoS ONE
Popis: The morphology and physiology of diaspores play crucial roles in determining the fate of seeds in unpredictable habitats. In some genera of the Brassicaceae different types of diaspores can be found.Lepidium appelianumproduces non-dormant seeds within indehiscent fruits while inL. campestredormant seeds are released from dehiscent fruits. These different diaspore types offer an excellent model system to analyse the allocation of relevant defence compounds into different tissues, which may maximise diaspore fitness. Total glucosinolate concentration and composition were analysed in immature and mature seeds and pericarps ofL. appelianumandL. campestreusing high-performance liquid chromatography. Moreover, transgenic RNAiL. campestrelines were used for comparison that produce indehiscent fruits due to silencing ofLcINDEHISCENCE, theINDEHISCENCEortholog ofL. campestre. Total glucosinolate concentrations were lower in green compared to mature seeds in all studiedLepidiumspecies and transgenic lines. In contrast, indehiscent fruits ofL. appelianummaintained their total glucosinolate concentration in mature pericarps compared to green ones, while in dehiscentL. campestreand in indehiscent RNAi-LcIND L. campestrea significant decrease in total glucosinolate concentrations from green to mature pericarps could be detected. Regarding the distribution of glucosinolate classes, high concentrations of 4-methoxyindol-3-ylmethyl glucosinolate were found in mature seeds ofL. appelianum, while no indole glucosinolates were detected in mature diaspores ofL. campestre. The diaspores of the latter species may rather depend on aliphatic and aromatic glucosinolates for long-term protection. The allocation patterns of glucosinolates correlate with the morpho-physiologically distinct fruits ofL. appelianumandL. campestreand may be explained by the distinct dispersal strategies and the dormancy status of both species.
Databáze: OpenAIRE
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