Method for toxicity test of titanium dioxide nanoparticles in ciliate protozoanTetrahymena

Autor:	Asad Ud-Daula, Gerd Pfister, Karl-Werner Schramm
Rok vydání:	2013
Předmět:	Titanium Environmental Engineering Cell growth Tetrahymena General Medicine Vacuole Biology biology.organism_classification Endocytosis Exocytosis Acute toxicity Cell biology Toxicity Tests Toxicity Nanoparticles Viability assay
Zdroj:	Journal of Environmental Science and Health, Part A. 48:1343-1348
ISSN:	1532-4117 1093-4529
Popis:	Titanium dioxide (TiO2) nanoparticles have a high surface-to-mass ratio and rapidly aggregate in water causing great difficulties for toxicity test exposed to aquatic organisms or other cell lines. This study uses a cell viability kit for routine toxicity test of TiO2 as well as other nanoparticles which accumulate in the aquatic environment. Tetrahymena immediately endocytoses TiO2 nanoparticles and stores them in food vacuoles until the particles undergo exocytosis as larger aggregates. However, during the process of endocytosis and exocytosis, TiO2 particles interfere with cell growth and consequently induce acute toxicity. It exerted high cell growth inhibition at 20 h incubation and induces significant cytotoxic effects. Surprisingly, the effect of TiO2 decreases at 40 h incubation, due to the recovery of cell growth and reduction of the cytotoxicity of the particles.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c67f7717da4c3f5b7c802ba32388e8f https://doi.org/10.1080/10934529.2013.781878 Zobrazit plný text záznamu