Identification of novel striatal genes by expression profiling in adult mouse brain
| Autor: | Muriel Koch, Jérôme A.J. Becker, D. Demebele, C. Bole-Feysot, A. Ghate, Dominique Filliol, Katia Befort, Brigitte L. Kieffer, B. Jost |
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| Přispěvatelé: | Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I, Institut Clinique de la Souris (ICS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Strasbourg (UNISTRA), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
DNA
Complementary Receptors Retinoic Acid Nerve Tissue Proteins Striatum Biology Transcriptome Mice 03 medical and health sciences MESH: Gene Expression Profiling 0302 clinical medicine MESH: In Situ Hybridization MESH: RNA MESH: Mice Inbred C57BL Complementary DNA MESH: Reverse Transcriptase Polymerase Chain Reaction Gene expression Animals MESH: Animals MESH: Brain Chemistry MESH: Nerve Tissue Proteins Gene MESH: Mice In Situ Hybridization Oligonucleotide Array Sequence Analysis 030304 developmental biology Brain Chemistry Genetics MESH: Receptors Retinoic Acid 0303 health sciences Expressed sequence tag Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling General Neuroscience [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology MESH: DNA Complementary Cell biology Mice Inbred C57BL Neostriatum Gene expression profiling nervous system MESH: Neostriatum Data Interpretation Statistical MESH: Oligonucleotide Array Sequence Analysis Gene chip analysis RNA MESH: Data Interpretation Statistical 030217 neurology & neurosurgery |
| Zdroj: | Neuroscience Neuroscience, Elsevier-International Brain Research Organization, 2007, 146 (3), pp.1182-92. ⟨10.1016/j.neuroscience.2007.02.040⟩ |
| ISSN: | 0306-4522 1873-7544 |
| Popis: | Large-scale transcriptome analysis in the brain is a powerful approach to identify novel genes of potential interest toward understanding cerebral organization and function. We utilized the microarray technology to measure expression levels of about 24,000 genes and expressed sequence tags in mouse hippocampus, frontal cortex and striatum. Using expression profile obtained from whole brain as a reference, we categorized the genes into groups of genes either enriched in, or restricted to, one of the three areas of interest. We found enriched genes for each target area. Further, we identified 14 genes in the category of genes restricted to the striatum, among which were the orphan G protein-coupled receptor GPR88 and retinoic acid receptor-beta. These two genes were already reported to be selectively expressed in the striatum, thus validating our experimental approach. We selected 6 striatal-restricted genes, as well as 10 striatal-enriched candidates, that were previously undescribed. We analyzed their expression by in situ hybridization analysis in the brain, and quantitative RT-PCR in both brain and peripheral organs. Two of these unknown genes displayed a notable expression pattern. The striatal-restricted gene H3076B11 shows uniform expression throughout and uniquely in the striatum, representing a genuine striatal marker. The striatal-enriched gene 4833421E05Rik is preferentially expressed in the rostral striatum, and is also abundant in kidney, liver and lung. These two genes may contribute to some of the many striatal-controlled behaviors, including initiation of movement, habit formation, or reward and motivation. |
| Databáze: | OpenAIRE |
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