Comparison of 99mTc-DTPA and urea for measuring cefepime concentrations in epithelial lining fluid
| Autor: | Sam Bayat, F Fraisse, A Rahoui, Francis Grimbert, P M Sorin, M Tod, O Petitjean, Daniel Anglade, B Verdière, K. Louchahi |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine Pathology medicine.medical_specialty medicine.drug_class Cefepime Antibiotics Biological Availability Biological Transport Active Lung injury Pharmacology Sensitivity and Specificity Epithelium chemistry.chemical_compound Dogs medicine Animals Urea Infusions Intravenous Analysis of Variance Lung medicine.diagnostic_test business.industry Blood–air barrier Pneumonia respiratory system Cephalosporins Disease Models Animal medicine.anatomical_structure Bronchoalveolar lavage chemistry Permeability (electromagnetism) Technetium Tc 99m Pentetate Female Radiopharmaceuticals business Bronchoalveolar Lavage Fluid medicine.drug |
| Zdroj: | European Respiratory Journal. 24:150-156 |
| ISSN: | 1399-3003 0903-1936 |
| DOI: | 10.1183/09031936.04.00106803 |
| Popis: | The efficacy of antimicrobial agents against pulmonary infections depends on their local concentrations in the lung. The aims of the present study were to: 1) compare technetium-99m diethylenetriaminepenta-acetic acid (99mTc-DTPA) and urea as markers of epithelial lining fluid (ELF) dilution for measuring ELF concentrations of pharmaceuticals; 2) quantify ELF cefepime concentrations in normal and injured lung; and 3) measure the increase in permeability to cefepime following oleic acid-induced acute lung injury. A modified bronchoalveolar lavage technique, based on equilibration of infused 99mTc-DTPA, was used to measure ELF volume. Cefepime was administered intravenously at steady plasma levels. Six serial bronchoalveolar lavages were performed 5 h after the beginning of infusion. ELF to plasma cefepime concentration ratios were 95 +/- 17 and 100 +/- 14.5% in normal and injured lung respectively. When urea was used as marker, cefepime concentration ratios were underestimated at 16.4 +/- 2.7 and 73.9 +/- 8.4% respectively. Cefepime blood/ airspace clearance increased from 3.8 +/- 0.7 micro x min(-1) in controls to 39.8 +/- 4.9 microL x min(-1) in acute lung injury. It was concluded that: 1) cefepime concentrations in epithelial lining fluid were in equilibrium with those in plasma in both normal and injured lung after 5 h at steady plasma concentrations; 2) epithelial lining fluid cefepime concentration by the urea method was much less underestimated in injured versus normal lung; and 3) acute lung injury induces a 10-fold elevation of cefepime blood/airspace clearance. |
| Databáze: | OpenAIRE |
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