The human telomeric nucleosome displays distinct structural and dynamic properties
| Autor: | Nikolay Korolev, Hsiang Ling Teo, Nikolay V. Berezhnoy, Aghil Soman, Daniela Rhodes, Vincent Olieric, Lars Nordenskiöld, Chong Wai Liew |
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| Přispěvatelé: | School of Chemical and Biomedical Engineering, School of Biological Sciences |
| Rok vydání: | 2020 |
| Předmět: |
Models
Molecular Cell division AcademicSubjects/SCI00010 Science Chromatin and Epigenetics Biology Crystallography X-Ray 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Molecular level Genetics Nucleosome Humans Gene Regulation 030304 developmental biology 0303 health sciences Gene regulation Chromatin and Epigenetics DNA Telomere Chromatin Nucleosomes Telomeric chromatin chemistry Telomeric dna Biophysics 030217 neurology & neurosurgery |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 |
| Popis: | Telomeres protect the ends of our chromosomes and are key to maintaining genomic integrity during cell division and differentiation. However, our knowledge of telomeric chromatin and nucleosome structure at the molecular level is limited. Here, we aimed to define the structure, dynamics as well as properties in solution of the human telomeric nucleosome. We first determined the 2.2 Å crystal structure of a human telomeric nucleosome core particle (NCP) containing 145 bp DNA, which revealed the same helical path for the DNA as well as symmetric stretching in both halves of the NCP as that of the 145 bp ‘601’ NCP. In solution, the telomeric nucleosome exhibited a less stable and a markedly more dynamic structure compared to NCPs containing DNA positioning sequences. These observations provide molecular insights into how telomeric DNA forms nucleosomes and chromatin and advance our understanding of the unique biological role of telomeres. Ministry of Education (MOE) Published version MOE2012-T3-1-001 MOE2018-T2-1-112 |
| Databáze: | OpenAIRE |
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