Comparative mitogenic and galactopoietic effects of IGF-I, IGF-II and DES-3-IGF-I in bovine mammary gland in vitro
| Autor: | I. Peri, M.F. McGrath, Arieh Gertler, A. Shamay, Robert J. Collier |
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| Rok vydání: | 1992 |
| Předmět: |
Cholera Toxin
medicine.medical_specialty medicine.medical_treatment Mammary gland Biology Organ culture Mammary Glands Animal Organ Culture Techniques Insulin-Like Growth Factor II Somatomedins Epidermal growth factor Internal medicine medicine Animals Lactation Insulin-Like Growth Factor I Receptor Cells Cultured Growth factor DNA Cell Biology Lipids Somatomedin Peptide Fragments Prolactin medicine.anatomical_structure Endocrinology Insulin-like growth factor 2 biology.protein Cattle Female |
| Zdroj: | Cell Biology International Reports. 16:359-368 |
| ISSN: | 0309-1651 |
| Popis: | Insulin-like growth factors (IGFs) I and II (IGF-I, IGF-II) and Des-3-IGF-I at physiological concentrations are potent mitogens of bovine undifferentiated mammary epithelial cells cultured in collagen in a serum-free medium. Des-3-IGF-I was found to be as potent as IGF-I, while IGF-II was significantly less active. All three factors acted either synergistically or additively with epidermal growth factor (EGF), cholera toxin and fetal calf serum (FCS). Indirect evidence indicates that despite its lower mitogenic activity the action of IGF-II is mediated through IGF-I receptors. The galactopoietic activity of Des-3-IGF-I and IGF-II was studied in an organ culture of bovine lactating mammary glands using lactogen-responsive fat synthesis as a test. Neither Des-3-IGF-I nor IGF-II exhibited galactopoietic activity nor did they affect the galactopoietic activity of prolactin. |
| Databáze: | OpenAIRE |
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