Effect of fetal bovine serum on 3-methylcholanthrene-induced transformation of hamster cells in vitro
| Autor: | R. J. Pienta, J. A. Poiley, R. F. Schuman, W. B. Lebherz |
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| Rok vydání: | 1979 |
| Předmět: |
Drug Evaluation
Preclinical Hamster Plant Science Biology medicine.disease_cause chemistry.chemical_compound Cricetinae Culture Techniques medicine Animals Lung Cloning Embryo Fetal Blood Molecular biology In vitro Clone Cells Transformation (genetics) Cell Transformation Neoplastic chemistry Methylcholanthrene Cattle Carcinogenesis Cell Division Fetal bovine serum Biotechnology |
| Zdroj: | In Vitro. 15:730-735 |
| ISSN: | 1475-2689 0073-5655 |
| DOI: | 10.1007/bf02618253 |
| Popis: | Eighteen lots of fetal bovine serum were tested for their ability to support clonal growth and 3-methylcholanthrene-induced morphological transformation of hamster embryo cells in vitro. Most of them supported cloning efficiencies of over 11%. However, cloning efficiency alone was an inadequate criterion for selecting serum for transformation studies, since no transformation was observed with some lots, even though their cloning efficiencies were over 16%. This shows the importance of pretesting serum for its ability to support morphological transformation before it is used in mammalian cell carcinogenesis tests. |
| Databáze: | OpenAIRE |
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