Report on the 19th International Society of Blood Transfusion Platelet Immunology Workshop 2018

Autor:	Gwen Clarke, Antoine Lewin, Lynnette Beaudin, Lucie Richard, Lynne Meilleur, Shadhiya Al Khan
Rok vydání:	2020
Předmět:	Blood Platelets endocrine system Blood transfusion medicine.drug_class medicine.medical_treatment Human leukocyte antigen 030204 cardiovascular system & hematology Immunologic Tests Monoclonal antibody Sensitivity and Specificity 03 medical and health sciences 0302 clinical medicine Antigen Isoantibodies Medicine Humans Platelet Antigens Human Platelet Blood Transfusion Genotyping biology business.industry Integrin beta3 Hematology General Medicine medicine.disease Immunology Neonatal alloimmune thrombocytopenia biology.protein Antibody business Laboratories hormones hormone substitutes and hormone antagonists 030215 immunology
Zdroj:	Vox sanguinisReferences. 115(8)
ISSN:	1423-0410
Popis:	Background and objectives The aims of the 19th International Society of Blood Transfusion Platelet Immunology Workshop were to compare the sensitivity and specificity of in-house and commercially available methods for the detection of alloantibodies against human platelet antigens. Survey regarding laboratory management of samples collected for the diagnosis of foetal neonatal alloimmune thrombocytopenia was also conducted. Materials and methods Twenty-nine laboratories from 17 countries were invited to participate. Seven serum or plasma samples for antibody identification and eight DNA samples for genotyping were sent to participating laboratories. Additionally, samples, critical reagents, materials and instructions for three exercises, one using a commercial kit (Pak Lx), one on platelet preparation for the detection of anti-HPA-3 antibodies and one for testing four anti-CD109 monoclonal antibodies for anti-HPA-15 antibody detection, were provided. Results Anti-HPA-1a, anti-HPA-2b, anti-HPA-5b and anti-GPIV were detected by the majority of the 28 reporting laboratories using their respective in-house MAIPA assay and/or a commercially available assay. Conversely, very few laboratories correctly identified anti-HPA-3a and HPA-15b. DNA genotyping of HPA and HLA alleles was highly accurate, with just a few discrepancies relative to the expected results. The Pak Lx kit has proven reliable for detecting anti-HPA-1a, anti-HPA-5a and anti-HLA; however, it failed at identifying an anti-HPA-3a in a clinical sample. Conclusions Some anti-platelet alloantibodies are reliably and consistently detected, yet others remain difficult to detect. Genotyping of HPA and HLA alleles has proven to be highly accurate and robust. Future work should focus on optimizing the detection of anti-HPA-3 and anti-HPA-15 antibodies.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c5fbfc80c47caec75e5abac8ea307f6 https://pubmed.ncbi.nlm.nih.gov/32468604 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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