Ionic strength for tailoring the synthesis of monomodal stealth cationic liposomes in microfluidic devices
| Autor: | Amanda da Costa e Silva de Noronha Pessoa, Gabriel Perli, Tiago A. Balbino, Lucimara Gaziola de la Torre |
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| Rok vydání: | 2018 |
| Předmět: |
Materials science
Chemical Phenomena Microfluidics Dispersity Kinetics 02 engineering and technology 01 natural sciences Micelle Polyethylene Glycols chemistry.chemical_compound Colloid and Surface Chemistry Cations Lab-On-A-Chip Devices 0103 physical sciences Zeta potential Cationic liposome Physical and Theoretical Chemistry 010304 chemical physics Phosphatidylethanolamines Osmolar Concentration Surfaces and Interfaces General Medicine 021001 nanoscience & nanotechnology Chemical engineering chemistry Ionic strength Liposomes 0210 nano-technology Ethylene glycol Biotechnology |
| Zdroj: | Colloids and surfaces. B, Biointerfaces. 179 |
| ISSN: | 1873-4367 |
| Popis: | In this work, we describe a hydrodynamic flow-focusing microfluidic process to produce stealth cationic liposomes (SCL), stabilized with poly(ethylene glycol) (PEG), with uniform and reproducible features. Through cryogenic transmission electron microscopy (cryo-TEM) characterization and real-time monitoring, we verified the formation of multi-sized lipid self-aggregates, which can be attributed to micelles formation. These structures tend to undergo deposition within the PDMS/glass microchannels through intermolecular interactions with the glass walls, hindering not only the process reproducibility but also the final biological application of the SCL products. In view of this, we propose the modulation of ionic strength of the side streams aiming to ionically shield the glass surface, decrease the intermolecular interactions of the lipid polar heads, and, essentially, to promote the bilayer-driven self-assembly of SCL with 1% of DSPE-PEG2000 lipid. Herein, we applied phosphate-buffered saline (PBS) from 10 to 50 mM concentration as side streams, and evaluated its effects on SCL final physicochemical properties in terms of size distribution, mean diameter, zeta potential and polydispersity index (PDI). We present evidences indicating that the ionic strength can be used as a microfluidic process parameter to modulate the lipids self-assembly kinetics whilst preventing micelles formation. Finally, the proposed diffusion-based microfluidic system with high ionic strength enables the formation of monodisperse (PDI |
| Databáze: | OpenAIRE |
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